To observe collagen fibers, samples can be stained with Masson trichrome (MT), Elastica van Gieson (EVG), or Elastica Masson (E-M); however, distinction between collagen fiber types I and III is not possible with this method. In drug discovery research, quantitative evaluation of the collagen fibers is required, so it is necessary to be able to distinguish between collagen fiber types I and III.

Access the complimentary app note to learn more about:

• Problems with polarized light observation of collagen fibers
• Advantages of high luminosity LED
• Quantitative evaluation of collagen fibers