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Improving ELISA Workflows with INTEGRA’s Pipetting Solutions

A wide range of liquid handling solutions are available to guarantee consistency and reproducibility


ELISAs (enzyme-linked immunosorbent assays) are popular plate-based methods to detect and quantify peptides, proteins, antibodies, or hormones. However, they generally involve multiple pipetting steps that are time consuming and tedious to perform, requiring consistent processing to ensure success. INTEGRA Biosciences offers a range of liquid handling solutions to improve ELISA workflows, designed to make your lab life easier and your results more reproducible.

The VIAFLO 96/384 handheld electronic pipette can vastly improve ELISA workflows by enabling the transfer of samples and reagents into 96 or 384 wells simultaneously. All the steps needed to run the protocol can be saved as a custom program on the instrument, which then guides the user with easy to follow prompts. This streamlines the workflow and eliminates user variability. And for even higher throughput, it can quickly fill multiple plates using the Repeat Dispense mode.

If you’re ready for a more walk-away approach, the ASSIST PLUS pipetting robot can automate the ELISA process—just choose the VIALAB program and steps you need, place the appropriate labware on the deck, and let the robot do all the work. Pipetting settings and assay set-up options can easily be modified in VIALAB, making sure the system is always perfectly suited to your assay. Various sample and tube types, and multiple reagents can also be accommodated on the deck, and the choice of pipetting options is huge—you can mount any of INTEGRA’s 25 electronic pipettes onto the ASSIST PLUS.

These solutions are partnered with INTEGRA’s Low Retention GripTips, perfect for reducing the residual tip volumes when pipetting buffers that contain surfactants, such as Tween 20. This combination of flexibility and automated, optimized processing helps to guarantee the consistency and reproducibility of your ELISAs. Visit the INTEGRA Biosciences website to see videos explaining how each platform can help your ELISA workflow.

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