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Resource Guide

How to Improve Imaging Workflows for Western Blots

Selecting the right biomolecular imager for your workflows increases efficiency and data quality

Successful detection and imaging of Western blots requires the combined use of an accurate protein detection technique and imaging device. While X-ray film and digital imaging solutions (e.g., charge-coupled device (CCD) camera-based imagers and scanner-based systems) transformed western blot detection, selecting the right imaging solution is critical to success and depends on the protein detection method and the advantages and limitations of each solution.

This resource guide on biomolecular imagers explores current requirements of chemiluminescence and fluorescence detection, including enhanced sensitivity, resolution, and data security, and their solutions. In particular, it provides valuable advice to:  

  • Work more efficiently with the right imager, including optimizing exposure times, automating signal-to-noise optimization, and scheduling
  • Improve image resolution, quality, and confidence for immunoblots as well as other research applications, including cell analysis, host cell protein ELISAs, and DNA gels
  • Extend dynamic range and avoid saturation
  • Ensure adherence to GxP, FDA 21 CFR Part 11, and EU GMP Annex 11 regulations for imaging workflows, including special focus on traceability, authenticity, audit trails, and user access.
  • Consolidate imaging equipment for multiple applications.

Download this guide now for in-depth resources, data, real-world examples, and insights to help you streamline your imaging workflows.

Work more efficiently, freeing valuable resources to drive your research forward, faster

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