It is recommended that the tip manufacturer proves that the system (Pipette-
Tip combination) meets the requirements and maximum permissible errors of
? Tips are single use – they shall not be cleaned and reused
? Attach the tip only once – it may be used for several replicates once it has
? Multi-channel pipette tips have maximum tolerance for varying in spacing
from their nominal axes – new limit for <100 µL volume pipette tips
and the Pipette-Tip System
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1. Manufacturer and model name
of the pipette(s) for which the
tips are proved to ful?ll the
2. Chemical compatibility and
3. Nominal volume
The Pipette and Tip Make One System
ISO 8655:2022 states that a pipette and a tip are considered as one system.
Every Pipette-Tip combination must be tested and shown to meet the
requirements described in the standard.
Use Sartorius tips with Sartorius
Tip manufacturers do not always
notify pipette producers about
changes to their tips. For the
most reliable performance, it is
best to use Sartorius tips with
Maximum permissible errors
ISO 8655 standard speci?es accuracy and precision limits as both
absolute (μl) and relative (%) values. To work according to the standard,
all instruments must meet the minimum performance limits.
Limits (random and systematic errors) are given for 100%, 50%, and
10% of the nominal volume.
Multi-channel pipettes have their own table - notice the di?erent
limits compared to single channel pipettes!
1. Usable volume range, basis of
adjustment (Ex), and maximum
permissible error limits
2. List of tips that can be used with
3. Suggested basis of routine testing
4. Information on care, cleaning,
sterilization, and, upon request,
Electronic motorized air displacement pipettes are considered in ISO
8655:2022 Part 2.
? Recognizes variety of operating modes
? Speed setting should be visible in the calibration certi?cate
? Method for testing multi-dispensing mode (in addition to pipetting mode)
ISO 8655:2022 contains updated requirements for producing and in-use control of piston-operated volumetric
apparatus (POVA), replacing the 1st edition from 2002. ISO 8655:2022 consists of nine parts in total. Here, we
describe the main points about Part 2: Pipettes
ISO 8655:2022 recognizes the mode and
speed settings of electronic pipettes.
Every Pipette-Tip combination must
be tested and shown to meet the
requirements and maximum permissible
errors described in ISO 8655:2022.
your Lab Environment
with Versatile Pipette
Webinar: How Does the
Revision of ISO 8655
A?ect the Quality Control
of My Pipettes?
Blog: Five Things You
Should Know about the
New Pipette Calibration
Infographic: Tips and
Standards for Pipette
Calibration with ISO8655
Calibration and Testing of
POVA acc. to Part 6 and 7
of ISO 8655: 2022