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Increasing Photostability and Target Specificity for Lateral Flow Assays and Western Blots

Conjugated polymer nanoparticles and optimized imaging systems boost lateral flow assay development with new capabilities

Lateral flow assays are an area of intense research and have led to highly robust and sensitive products, proving themselves indispensable for rapid diagnostics. Advancements in technologies used for assay development drive similar progress in neighboring applications including Western Blot. In particular, conjugated polymer nanoparticles, a new class of molecular bioimaging probes, strengthens sensitivity and specificity with bright, photostable signals across a wide range of fluorescence emission wavelengths (420 to 1000 nm), enabling multiplexing. 

Equally important in assay development is a sensitive and flexible imaging system optimized for detection. Imaging systems with multiple lights and filters for colorimetric or fluorescence imaging across the spectrum, including UV, RGB, and IR long and short wavelengths, and the ability to accommodate custom, third-party filters provide the greatest flexibility and value for users. 

Download this whitepaper to learn more about how to improve lateral flow assay and Western Blotting methodologies with conjugated polymer nanoparticles and an ideal imaging system, including: 

  • Saving time with increased sensitivity that enables primary antibody labeling in Western blots.
  • Simultaneous direct imaging of both excitation and emission signals for markers.
  • Evaluating sensitivity, dynamic range, and limits of detection for use of such fluorophores in lateral flow diagnostic assay development.
Application Note Cover: ImageQuant™ 800 imager: using dyes across the spectrum

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