scientists with cell culture medium

© Lonza, Basel

Lonza Launches New TheraPEAK™ SfAAV™ Medium to Boost and Optimize the Production of AAV in Sf9 Insect Cells

TheraPEAK™ SfAAV™ Medium is specifically designed to support the development of safe, scalable, life-saving gene therapies

| 2 min read
Register for free to listen to this article
Listen with Speechify
0:00
2:00

BASEL, SWITZERLAND — March 23, 2020 — Lonza announces the launch of the TheraPEAK™ SfAAV™ Medium, the first chemically defined, non-animal origin medium designed specifically for the production of Adeno Associated Virus (AAV) in Spodoptera Fuigiperda (Sf9) insect cells for gene therapy applications. The new high-performance medium accelerates cell growth, increases productivity, and reduces process variability and costs, expediting time-to-market for safe, scalable, life-saving gene therapies. The unique benefits of the TheraPEAK™ SfAAV™ Medium, which marks the latest addition to Lonza’s TheraPEAK™ Family of cell culture media and reagents for cell and gene therapy applications, are summarized in an informative poster that can be downloaded for free from the Lonza website.

Lonza's TheraPEAK™ SfAAV™ Medium.
© Lonza, Basel

Gene therapy holds great promise for the treatment of rare disorders and diseases, and viral vectors are commonly used to facilitate the delivery of the gene of interest into patient cells. AAV has been established as a viral vector of choice, due to not replicating in patients, thus posing a lower health risk. Owing to their ability to be grown at high densities, the Sf9 insect cells are ideal for use as hosts for the production of large AAV quantities. To date, however, translational scientists and researchers have been challenged with the lack of a medium dedicated to AAV production in Sf9 insect cells.

Lab manager academy logo

Get training in Lab Crisis Preparation and earn CEUs.

One of over 25 IACET-accredited courses in the Academy.

Certification logo

Lab Crisis Preparation course

"Biotechnology companies are under increasing pressure to fast track the development of safe gene therapies to treat a range of life-threatening conditions," said Amber Jones, product manager, Lonza. "To achieve this, they need to create and implement reliable and consistent GMP-scalable processes, ensuring that product quality and patient safety always remain at the forefront. The new TheraPEAK™ SfAAV™ Medium has been designed to allow for increased AAV production in Sf9 insect cells, reduced downstream purification, and easier regulatory approval, streamlining process scale-up for the commercialization of gene therapies."

Lonza’s TheraPEAK™ SfAAV™ Medium has been explicitly designed to address this need, providing a cost and time efficient solution for the production of AAV in Sf9 insect cells. Allowing for rapid cell growth, the TheraPEAK™ SfAAV™ Medium reduces processing time significantly, enabling cell infection one day earlier than similar media available on the market, and boosting laboratory performance. Due to its chemically defined nature, the new hydrolysate free medium produces AAV that requires less purification, further decreasing the overall processing time and minimizing labor requirements. Furthermore, the TheraPEAK™ SfAAV™ Medium supports consistent cell growth throughout all phases of the culturing process, considerably reducing process variability.

As a chemically defined, non-animal origin product, the TheraPEAK™ SfAAV™ Medium is safer to use than media containing animal or human components, thereby facilitating regulatory compliance. Additionally, the medium is supplied with a U.S. Food and Drug Administration drug master file, alleviating the relevant preparation and submission burden.

Poster: Evaluation of AAV Production in Insect Cells Using Chemically Defined Media

A new poster developed by Lonza’s cell culture experts discusses the results of a study whereby the TheraPEAK™ SfAAV™ Medium was used to support the production of AAV in Sf9 insect cells. The study demonstrated the ability of the new medium to ensure lot-to-lot consistency, stable doubling time and increased AAV production.

The poster is free to download and can be accessed via the following link: https://bioscience.lonza.com/download/content/asset/36112

Interested in life sciences?

Subscribe to our free Life Sciences Newsletter.

Is the form not loading? If you use an ad blocker or browser privacy features, try turning them off and refresh the page.

Further information about the TheraPEAK™ SfAAV™ Medium can be found via www.lonza.com/therapeak-sfaav 

Loading Next Article...
Loading Next Article...

CURRENT ISSUE - January/February 2025

Energizing Leadership in Action

The science-backed behaviors that help leaders inspire thriving teams and organizations

Lab Manager January/February 2025 Cover Image