PCR is a technique used to amplify DNA strands by cycling through specific temperatures to facilitate different stages of the reaction. Building on this, qPCR allows the quantification of DNA strands in real time. When purchasing technology for PCR and qPCR, consider the accuracy of the temperature and fluorescence measurements, and the robustness of the instrument as it will be running for long periods of time.
In the eBook you will learn:
- Questions to ask when buying PCR technology
- Deciding on a PCR detection method
- Optimizing and troubleshooting PCR
- Speed and sensitivity give qPCR the leg up in certain clinical cases
- Designing qPCR assays
