DPX recently published a new application note with Hamilton Robotics titled “Automated Method for High-Throughput LC-MS/MS Quantitation of Testosterone from Serum: An Improved Validated Method” where a new ultra pure grade filtration tip was introduced. The method for the extraction of testosterone from serum used Low Porosity Filtration Tips- Ultra Pure (LPFT-UP) and incorporated a ZnSO4 solution in addition to acetonitrile on a Hamilton Heater Shaker (HHS) for improved recovery and precision during protein precipitation. The LPFT-UP provide a high purity filtration media for methods with very low detection limits.
Patient samples had a calculated recovery of testosterone at 99.5 percent with no detected matrix effects. This demonstrates that the use of ZnSO4 and acetonitrile is very efficient in extracting testosterone, and the use of ultra pure grade tips (LPFT-UP) resulted in negligible losses. The introduction of Low Porosity Filtration tips- Ultra Pure provides a new product for low detection level methods.
“We are really excited to expand our product offering for steroid testing,” said Carmen Adamson, brand manager. DPX plans to expand on the research and development to include a full panel of steroids. “Testosterone imbalance can signal a problem in both men and women. This method provides an accurate and precise tool to aid in that determination,” said Evan DiVirgilio, application scientist. DPX continues to provide methods for efficient sample processing for clinical and forensic laboratories.
DPX Technologies manufactures patented sample preparation products and specializes in custom workflows for a diverse client base including clinical, forensic, food safety, and pharmaceutical industries. Their products offer INTip™ sample preparation solutions that are compatible with semi-automated and fully automated liquid handling systems. This enables high sample throughput and high laboratory efficiency.