How it Works Webinar
Tuesday, June 24, 2025
1 PM ET
Synthetic oligonucleotides are increasingly central to modern research and therapeutic development—but their analysis via LC/MS often relies on large volumes of hazardous mobile phase additives like HFIP and TEA. HFIP, a PFAS-class compound, is particularly concerning due to its environmental persistence and negative impacts on human health. As sustainability becomes a growing priority across analytical labs, there’s a clear need for methods that reduce solvent and modifier use without compromising data quality.
In this webinar, scientists from Axcend and Agilent Technologies will present a collaborative study demonstrating how a standard oligonucleotide LC/MS method was successfully transferred to a compact, capillary-scale system. The result: a >99% reduction in HFIP, TEA, and methanol consumption, while delivering the expected chromatographic resolution and mass spectral accuracy.
As an attendee, you will learn more about:
- The environmental and operational challenges of PFAS-containing modifiers in LC/MS
- Method transfer strategies from standard to microflow platforms
- Data from oligonucleotide standards and the therapeutic siRNA Givosiran
- Practical implications for sustainability and system maintenance
Whether you're working in pharma, environmental testing, materials science, or any field where liquid chromatography plays a role, this session will offer practical guidance on greening your workflow without losing precision.
Speaker
Lee Bertram
Technical Product Manager
Agilent
Lee Bertram currently serves as Technical Product Manager for LC Triple Quadrupole Mass Spectrometers at Agilent Technologies. With over a decade of experience in analytical chemistry, Lee has held roles in both research and product development across biotech startups and global life sciences companies. His background includes extensive work in method development, GMP compliance, and LC/MS applications spanning quadrupole, triple quadrupole, and Q-TOF technologies.
Speaker
Samuel Foster, Ph.D.
Application Scientist
Axcend
Samuel Foster completed his Ph.D. in Pharmaceutical Chemistry from Rowan University in 2025. His research has focused on the development and application of capillary scale liquid chromatography instrumentation. He currently works at Axcend as an application scientist focusing on the development of chromatographic workflows for a variety of analyte classes including oligonucleotides, monoclonal antibodies, and drugs of abuse.
