Viruses are the scientific story of 2020 with the global COVID-19 pandemic thrusting them to the forefront of almost every person’s consciousness around the world. Development of antibodies to specific viruses form the core of future immune response. Vaccines can be complex and may contain many species that span a large size range from antibodies, virus fragments such as proteins and nucleic acids, and polysaccharide-protein complexes, up to large aggregates, often over 100 nm in diameter. This means such samples are challenging to separate and characterize using column-based chromatography techniques.

Access this white paper to learn how asymmetrical flow field-flow fractionation (AF4) techniques can help virus, vaccine, and antibody researchers as it is a stationary-phase-free separation technique for characterization of polydisperse samples from about 1 nanometer to 1 micron in diameter.