Virtually every molecular biology lab is familiar with the pain of traditional PCR preparation with its tedious and error-prone workflows that are challenging to scale. Add seemingly endless optimization cycles, and PCR workflows can easily dominate staff workloads. Choosing the right assays, tools, and technology can substantially improve PCR efficiency, accuracy, and reproducibility, enabling laboratories to manage higher throughput with consistent quality.
This resource compendium covers everything you need to know about PCR, from assays and methods to the tools that support any workflow. These resources provide expert insights to optimize your PCR workflows, detailing specific techniques, applications, and the advantages of automating at different scales.
Access the full collection now to learn more about:
- PCR’s fascinating history, its mechanism and different variations—including qPCR, RT-PCR, vPCR, and ddPCR—and techniques for troubleshooting common issues like poor specificity
- The simple PCR tips that can make or break your success
- How to design your PCR lab space to mitigate amplicon and sample contamination
- Selecting the best tools for your application needs and throughput
- The difference between dye-based and probe-based qPCR assays, and how to validate your experiments and analyze your data
- Overcoming common challenges like low yield and non-specific binding with better primer design
- Efficient automation protocols for magnetic bead-based PCR purification that produce high-yield and contaminant-free DNA
- Strategies to increase throughput and accuracy in sample transfers from microcentrifuge tubes to multi-well PCR plates
- Best practices for low-volume, high-precision liquid transfers
- Real-world application examples demonstrating how laboratories successfully integrate automated liquid handling solutions to streamline PCR setup, purification, and high-throughput assays
