Q: Conventional methods for immunological analyte detection are laborious, tedious, and low-throughput. How can I simplify my workflow while retaining sensitivity?

Immunoassays rely on the interaction between antibodies and the analyte of interest. Traditional methods used to perform immunological detection of analytes, such as ELISA (Enzyme-Linked Immunosorbent Assays) and Western blot techniques, frequently involve labor-intensive, multistep procedures. Western blots require multiple transfer, separation, and wash steps, which significantly limit the throughput of this method. Although ELISAs are somewhat more amenable to screening applications, they also involve sample transfer and multiple washing steps. Both of these methods are time-consuming to perform, requiring several hours or overnight incubations to complete. The number of steps involved can also lead to significant assay-to-assay variability.

A: Lumit™ Immunoassays are a simple, rapid alternative to conventional immunoassay approaches, offering a no-wash, bioluminescence-based method for analyte detection.

The homogeneous detection chemistry of Lumit™ Immunoassays provides several advantages over traditional immunoassay methods. These immunoassays offer sensitive luminescence detection with broad linear dynamic range, eliminating both the need for sample dilutions and the assay variability associated with transfers and multiple wash steps. The simple add-mix-read protocol requires no wash steps and can be completed in as little as 30–90 minutes, using only a standard plate reading luminometer. Everything is performed in-well, enabling direct analyte measurement within the cell culture plate or on medium removed from the cells, making them ideal for high-throughput applications.

Get training in Lab Crisis Preparation and earn CEUs.

One of over 25 IACET-accredited courses in the Academy.

Lab Crisis Preparation course

To learn more, visit promega.com/LumitTechnology