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2014-15 Product Resource Guide: HPLC Columns

Get fun facts about HPLC columns, a list of questions to ask when buying HPLC columns, the most recent HPLC column releases, and an updated HPLC column manufacturer's list in this section.

Top 5 Things You May Not Know About HPLC Columns

1. The first chromatography column was developed by the Russian botanist Mikhail Tsvet who, in 1901, washed an organic solution of plant pigments through a vertical glass column packed with an adsorptive material. He discovered that the pigments separated into a series of discrete colored bands on the column, divided by regions entirely free of color.

2. Column chromatography was popularized during the 1930s when the chemists Richard Kuhn and Edgar Lederer successfully used the technique to separate a number of biologically important materials.

3. In 1938, Harold C. Urey and T. I. Taylor developed the first ion exchange chromatography column based on a zeolite stationary phase. This technique allowed, for the first time, the separation of particles based on their charge.

4. In 1941, the concept of using water as a stationary liquid supported on inert silica in conjunction with a mobile chloroform phase was developed by two British chemists, Archer Martin and Richard Synge. Their design enabled the solute molecules to be partitioned between the stationary liquid and the mobile liquid phases, improving separation. Martin and Synge were instrumental in the development of increasingly sophisticated chromatographic techniques during the 1940s and 1950s.

5. In 1942, ion-exchange column chromatography was used to great effect during the Manhattan Project to separate elements such as uranium fission products produced by thermonuclear explosions.

For the Top 7 Questions to Ask When Buying HPLC Columns, click  here

Recently Released HPLC Columns

Cortecs 2.7 Micron HPLC Columns

  • Designed for analytical scientists who need to maximize performance on their existing LC systems
  • Run at lower pressures while delivering high efficiencies
  • Give the scientist the flexibility to use longer column lengths to improve resolution or higher flow rates to speed instrument analysis times and increase throughput
  • Available in C18+, C18, and HILIC chemistries


GlycanPac AXR-1 HPLC/UHPLC Columns for Glycan Analysis

  • Offers extremely high-resolution separations based on charge, isomerism, and size
  • Designed to facilitate both high resolution glycan profile characterization and quantification
  • Help users achieve greater resolution of glycans using HPLC, providing easier profile characterization
  • Also designed for excellent resolution of labeled and unlabeled glycans, and are compatible with fluorescence and mass spectrometry detection methods

Thermo Fisher Scientific

Kinetex Core-Shell Biphenyl HPLC/UHPLC Columns

  • Give researchers excellent orthogonal selectivity to traditional C18 phases
  • Suited for a broad range of complex-mixture analyses in clinical research and forensic toxicology, food and environmental testing, and pharmaceutical, bioanalytical, and DMPK studies
  • Deliver all the benefits inherent with Phenomenex’s Core-Shell technology including better resolution, higher efficiency, sharper peaks, and greater sensitivity than traditional HPLC/UHPLC media



  • Provide improved analysis of vitamins such as K3, K2, D2, D3, E, K1—saving up to 60% of analysis time for higher efficiency
  • Allow chromatographers to see increased resolution similar to sub 2 μm porous particles but with considerably lower back pressure
  • Allow users to achieve high column resolutions with short analysis times when using conventional HPLC


HPLC Column Manufacturers

Advanced Chromatography Technologies 

Agela Technologies 


Applied Biosystems 

Beckman Coulter 






ES Industries 

Grace Discovery Sciences 



J & K Scientific 

Life Technologies  


Peeke Scientific  








Thermo Fisher Scientific  

Tosoh Bioscience