Fluorescence and UV-Vis absorbance are complementary measurement techniques for the quantification and quality control of samples. Each method has advantages depending on the application and sample being quantified.
UV-Vis absorbance measures the amount of light absorbed by a sample at a specific wavelength, making it a quick and easy way to accurately quantify a wide range of samples. When using fluorescence assays, fluorophores bind to the sample of interest and are excited with a specific wavelength of light. This increases the specificity and sensitivity of measurements.
Download this eBook to learn more about the advantages of each method and when to employ absorbance, fluorescence, or both, courtesy of DeNovix