• The rationale for why the biosafety cabinet has been selected (sterility, biosafety, or a combination). This would include the risk assessment process that led to its selection for use.
• Aseptic technique and good microbiological work practices.
• Personal protective equipment (PPE) required for the proposed use of the biosafety cabinet, including how PPE should be donned and doffed.
• How the biosafety cabinet operates and what are the potential interferences of successful operation.
• How to use the biosafety cabinet, from turning it on, preparation for work, carrying out work, and how to terminate work and decontaminate the biosafety cabinet to prepare it for its next use.
• Maintenance of the biosafety cabinet.
• How to determine if the biosafety cabinet is operating adequately (checks of pressure gauges, odd noises, emanation of odors, etc.) and to whom they should be reported for investigation.

(Source: Karen Byers, President, American Biological Safety Association)