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2017 UV-VIS Spectrophotometer Survey Results

Find out readers’ top 10 uses and more from our latest UV-Vis spectrophotometer survey results.

Erica Tennenhouse, PhD

Erica Tennenhouse, PhD, is the managing editor of Clinical Lab Manager.

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Ultraviolet-visible (UV-Vis) spectrophotometry is arguably the most common as well as one of the oldest forms of absorption-based analysis. UV and visible regions of the electromagnetic spectrum are contiguous: UV wavelengths range from 10 to 4000 angstroms; visible wavelengths range from 4000 to 7000 angstroms

Top 5 Questions You Should Ask When Buying a UV-Vis Spectrophotometer

  1. For what applications will you be using the instrument for? This will help you determine the detection range you require. Don’t forget to consider future applications that may require a broader range
  2. What range of stray light performance are you comfortable with for your application and budget?
  3. Consider what sort of samples you’ll be working with in order to determine what absorbance range you will need in your UV-Vis spectrophotometer. For example, if it is a turbid or concentrated liquid or a solid sample that is optically thick, you may require a working absorbance range between 5 Å and 8 Å or higher.
  4. What level of throughput and reliability do you need?
  5. How much will the instrument cost? Don’t forget to factor in the cost of maintenance, etc. along with the cost of acquisition.

Types of UV-Vis spectrophotometers used by survey respondents:

Single beam66%
Dual beam47%
Array based11%

Top 10 UV-Vis spectrophotometer uses according to survey respondents:

Chemical Analysis52%
Biochemistry and biology21%
Color / dyes11%
Cell culture research9%
Food safety and testing analysis7%

Nearly 55% of respondents are engaged in purchasing a new UV-Vis spectrophotometer. The reasons for these purchases are as follows:

Replacement of an older spectrophotometer
Addition to existing systems
Need an instrument that provides faster acquisition and analysis of data
Need an instrument that is simple to operate and maintain
Need an instrument that provides excellent reproducibility
Need an instrument that has a broad range of accessories

Top 10 features/factors respondents look for when purchasing a UV-Vis spectrophotometer:

Excellent Reproducibility82%
Wavelength Accuracy75%
Better Sensitivity and Resolution69%
Ease of Maintenance/Low Operating Costs64%
Service/Support in General47%
Onsite Service/Support34%

For more information on UV-Vis spectrophotometers, including useful articles and a list of manufacturers, visit

See the most recent survey results