Top 7 Questions You Should Ask When Buying an Automated Liquid Handler
- What kind(s) of dispensing technology is used? Peristaltic pump dispensing offers low prime volumes and backflushing; microprocessor- controlled syringes have fast output and high precision. Hybrid detection systems combine both technologies in one and can even add washing functions.
- Is plate handling automatable? Manual plate handling can slow productivity. Automating the process with a compatible microplate stacker increases throughput with walk-away operation.
- Can it accommodate magnetic or plastic bead-based assays? If using bead-based assays, it should be equipped with appropriate magnets or vacuum filtration for critical wash steps.
- What is the volume range, and how many different sample vessel types may be used?
- Ask about the software—is it integrated and user-friendly? Does it allow for pre-programmed and custom protocols?
- What is the flow rate spectrum? A wide flow rate spectrum allows use with sensitive cell-based assays to viscous liquids.
- What assay validation data is available for this specific liquid handler? This provides proof that the instrument performs as indicated.
Types of automated liquid handling systems used by survey respondents:
| Stand-Alone | 54% |
| Individual benchtop workstations | 54% |
| Self-contained multi instrument systems | 28% |
Applications for automated liquid handling systems as reported by survey respondents:
| Serial dilution | 61% |
| Plate replication | 39% |
| Plate reformatting | 34% |
| PCR setup | 30% |
| High-throughput screening | 27% |
| Cell culture | 22% |
| Whole genome amplication | 14% |
| Array printing | 7% |
| High-density array printing | 3% |
| Other | 20% |
Nearly 30% of respondents are engaged in purchasing a new automated liquid handling system. The reasons for these purchases are as follows:
 | Replacement of an aging system | 24% |  |
 | Addition to existing systems, increase capacity | 43% | |
 | Setting up a new lab | 22% | |
 | First time purchase | 11% |
Top 10 features/factors respondents look for when purchasing an automated liquid handler:
| Accuracy and precision for a wide range of compatible fluids | 81% |
| Safe sample handling - No cross-contamination | 80% |
| Maintenance/service/availability of accessories and replacement parts | 62% |
| Rapid throughput | 49% |
| Price | 47% |
| Pipetting flexibility - Pipette with 384, 96, 24, 16, 12, or 8 tips | 41% |
| Maximum operator safety | 37% |
| Sample tracking | 34% |
| Size - small footprint | 30% |
| Ability to expand on instrument as requirements change | 29% |
For more information on liquid handlers, including useful articles and a list of manufacturers, visit www.labmanager.com/liquid-handling
