Image of a hand slipping into a blue latex laboratory glove illustrating the importance of glove inspection and removal

Glove Inspection, Use, Care and Removal

Once you've selected the ideal gloves for your application, you should inspect them for defects before entering into the action. You should check for punctures, signs of cracks, discoloration, etc.

Written byTrevor Henderson, PhD
Updated | 3 min read
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Gloves are an essential component of personal protective equipment (PPE) in laboratories, medical facilities, and various industrial environments. Proper glove use helps prevent contamination, protects against hazardous materials, and ensures the safety of both users and their surroundings. However, maintaining glove integrity and following proper procedures for inspection, use, care, and removal are critical for effective protection. Here are best practices for ensuring safe and efficient glove handling.


Glove Inspection, Use, and Care

  1. Inspect Gloves Before Use: Before using gloves, thoroughly inspect them for any signs of damage or degradation. Look for punctures, swelling, shrinking, cracking, or discoloration. To test for pinholes, inflate the glove by blowing or trapping air inside, then gently roll the glove to identify any leaks. Avoid testing by filling gloves with water, as this can weaken the material and compromise its effectiveness.

  2. Change Gloves at the First Sign of Contamination: Disposable gloves should be replaced immediately if there is any sign of contamination or damage. Long-term exposure to chemicals or repeated use can degrade the glove’s surface, reducing its protective capability. For reusable gloves, wash them frequently during extended use to maintain their effectiveness and prevent the buildup of hazardous substances.

  3. Minimize Cross-Contamination: While wearing gloves, avoid handling items not directly related to the task, such as phones, equipment, or wastebaskets. Be cautious about touching your face, hair, or clothing, as this can lead to cross-contamination and reduce the effectiveness of your PPE.

  4. Wash Gloves Before Removal: Before removing gloves, wash their exterior thoroughly to minimize the risk of spreading contaminants. After glove removal, wash your hands immediately with soap and water to ensure complete decontamination.

  5. Follow Manufacturer’s Care Instructions: For reusable gloves, adhere to the manufacturer’s care and cleaning instructions. Properly storing gloves in a clean, dry place extends their lifespan and maintains their protective properties. Single-use gloves should be disposed of in accordance with your facility’s waste management protocols to prevent environmental or biological hazards.


Proper Glove Removal Technique

Removing gloves safely is just as important as using them correctly to avoid direct contact with contaminants. Follow these step-by-step instructions to ensure safe glove removal:

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  1. Grasp the Exterior of One Glove: Using your opposite gloved hand, pinch the exterior of the glove near the wrist.

  2. Pull the Glove Off Inside-Out: Carefully pull the glove off your hand, turning it inside out so that any contamination is contained within the glove. Hold the removed glove in your other gloved hand.

  3. Ball the Removed Glove: Roll the first glove into a ball and keep it in your still-gloved hand.

  4. Insert an Unprotected Finger Under the Remaining Glove: Slide a clean, ungloved finger under the cuff of the remaining glove, being careful not to touch the exterior.

  5. Remove the Second Glove Inside-Out: Pull the second glove off, turning it inside out as well. The first glove should end up enclosed within the second glove, containing all contaminants.

  6. Dispose of Gloves Properly: Immediately discard the gloves in an appropriate waste container. Do not leave used gloves on surfaces or mix them with regular trash if they’ve been exposed to hazardous substances.


Frequently Asked Questions

1. How often should I inspect my gloves?

 Inspect your gloves before each use to check for any signs of damage or degradation, such as punctures, cracks, or discoloration. Regular inspections ensure optimal protection.

2. Can reusable gloves be washed and reused indefinitely?

 No, reusable gloves have a limited lifespan. Follow the manufacturer’s care instructions and replace gloves if they show signs of wear or after repeated use to maintain safety.

3. What should I do if my gloves come into contact with hazardous chemicals?

 If your gloves come into contact with hazardous chemicals, wash the exterior before removing them. Dispose of single-use gloves properly and wash your hands immediately.


Conclusion: Ensuring Safe and Effective Glove Use

Proper glove inspection, use, care, and removal are essential steps in maintaining a safe and contamination-free environment. By inspecting gloves for damage, minimizing cross-contamination, and following proper removal techniques, individuals can ensure optimal protection and safety. Whether in a laboratory, healthcare facility, or industrial setting, adhering to these best practices will help prevent accidents and safeguard both personnel and their workspaces.

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About the Author

  • Trevor Henderson headshot

    Trevor Henderson BSc (HK), MSc, PhD (c), has more than two decades of experience in the fields of scientific and technical writing, editing, and creative content creation. With academic training in the areas of human biology, physical anthropology, and community health, he has a broad skill set of both laboratory and analytical skills. Since 2013, he has been working with LabX Media Group developing content solutions that engage and inform scientists and laboratorians. He can be reached at thenderson@labmanager.com.

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