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New MALDI Imaging Applications Note

MALDI imaging is a powerful mass spectrometry technique available with JEOL’s MALDI Imaging SpiralTOF


(Peabody, Mass.)  JEOL USA has produced a new MALDI imaging technical applications note that describes the unique analytical process for a variety of biological and organic samples. MALDI imaging is a powerful mass spectrometry technique available with JEOL’s MALDI Imaging SpiralTOF. It is used to assess the distribution of proteins, peptides, lipids, drugs, and metabolites in tissue specimens, as well as compound distribution in organic materials. MALDI is an acronym for matrix-assisted laser desorption/ionization mass spectrometry. 

brain JEOL MALDI image of a mouse brain section with DHB matrix. JEOL’s MALDI Imaging SpiralTOF Mass Spectrometer offers unique capabilities that include imaging of samples with irregular surfaces. In a conventional reflectron time-of-flight mass spectrometer, the flight time is influenced by small changes in the target position. To maintain high mass-resolving power across an entire sample, the sample must be perfectly flat. However, it is impossible to achieve a perfectly flat surface for real-world samples. Because small variations in the sample surface are only a tiny fraction of the SpiralTOF’s 17-meter flight path, it is possible to maintain high mass-resolving power for the entire image.  This is critical if you need to know the accurate spatial distribution of a specific compound without interference from background interferences. 

High-resolution mass spectra are collected for the entire image in each of the examples ranging from lipids in mouse brain tissue to Organic Electroluminescent Panels and laser desorption ionization of ballpoint inks. 

The full-color applications note is available for free download at More than a dozen application-specific notes are also available on specific MALDI imaging applications on the JEOL website at