Bruker’s new nanoElute® nano-flow UHPLC system, together with the new Proteoform Profiling™ 1.0 solution, aims to bring enhanced ease-of-use to nano-spray mass spectrometry, which is essential for label-free discovery workflows on intact protein mixtures.
Professor Leonard Foster at the Department of Biochemistry & Molecular Biology of the University of British Columbia in Vancouver, Canada, stated: “LC-MS experts and biologists alike require trouble-free operation and ease-of-use, along with top performance. Our experience has shown that the nanoElute system readily meets those high demands.”
Both innovations take advantage of the superior performance of Bruker’s impact II and ETD-enabled maXis II UHR-QTOFs, which deliver accurate and reproducible proteoform profiles from complex intact, undigested protein mixtures. Unlike common top-down approaches, these UHR-QTOFs deliver information from proteoform mixtures with a reduced level of pre-fractionation, enabling the analysis of large sample sets from clinically relevant cohorts, or for longitudinal studies. The Proteoform Profiling 1.0 workflow automates the use of Bruker’s proprietary Dissect™ and SNAP™ algorithms, together with advanced molecular filtering functions, to generate a non-redundant list of monoisotopic masses, intensities and retention time from the isotopically resolved proteins. More…
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