Centrifugation and filtration have been widely accepted as techniques required for clarifying complex cell cultures to recover extracellular proteins such as monoclonal antibodies (mAbs). However, these steps can be time consuming and costly for labs growing their cultures in 24-well plates.

This scientific brief offers an alternative to the use of centrifugation/filtration/flocculation to clarify and sterilize mammalian cell cultures. It describes an assessment of a new 24-well clarification and sterile filtration plate and a 24-well sterilization-only filter plate for the recovery of proteins present in the supernatant.

Access the application note, courtesy of Pall Corporation.