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Reviving a Long-Extinct Bird

Researchers sequence the complete genome of the bush moa

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Using ancient DNA extracted from the toe bone of a museum specimen, Harvard biologists have sequenced the genome of an extinct, flightless bird called the little bush moa, shedding light on an unknown corner of avian genetic history.

Published in Science Advances, the work is the first complete genetic map of the turkey-sized bird whose distant living cousins include the ostrich, emu, and kiwi. It is one of nine known species of moa, all extinct for the last 700 years, which inhabited New Zealand before the late 1200s and the arrival of Polynesian human settlers.

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“We’re pulling away the veil across the mystery of this species,” said senior author Scott V. Edwards, professor in the Department of Organismic and Evolutionary Biology and curator of ornithology at the Museum of Comparative Zoology. “We can study modern birds by looking at them and their behavior. With extinct species, we have very little information except what their bones looked like and in some cases what they ate. DNA provides a really exciting window into the natural history of extinct species like the little bush moa.”  

Bush moa were the smallest species of moa, weighing about 60 pounds and distributed in lowland forests across the north and south islands of New Zealand. Genomic analysis has revealed their closest living relatives aren’t kiwis, as was originally speculated, but rather tinamous, a Neotropical bird group from which they diverged genetically about 53 million years ago.

The research offers new genetic evidence for various aspects of bush moa sensory biology. Like many birds, they had four types of cone photoreceptors in their retinas, which gave them not only color but also ultraviolet vision. They had a full set of taste receptors, including bitter and umami.

Perhaps the most remarkable trait of these flightless birds is their complete absence of forelimb skeletal elements that typically comprise birds’ wings, the researchers wrote. Studying the moa genome could offer new clues into how and why some birds evolved to become flightless.

The scientists used high-throughput DNA sequencing, which allows rapid sequencing of short DNA fragments. To produce the bush moa genome, the team sequenced the equivalent of 140 bird genomes, or about 140 billion base pairs of DNA, only about 12 percent of which was actual moa DNA (the rest was bacterial).

They then pieced together the genome, taking each snippet of DNA and mapping it to its correct position. Assembly of extinct species is painstaking work, which has gotten a big boost from technologies like high-throughput sequencing. Other species that have been mapped similarly are the passenger pigeon, the woolly mammoth, and our close relative, the Neanderthal. Using an existing emu genome as a guide, researchers strung together the bush moa’s genetic sequence by finding overlaps between each genetic snippet, essentially reconstructing a long puzzle of 140 billion pieces.

The project originated more than 15 years ago in the lab of the late Allan J. Baker. An expert in ancient bird DNA at the Royal Ontario Museum, Baker was the first to extract and sequence the bird’s DNA from a fossil recovered on the South Island of New Zealand.

Also involved in the initial DNA processing and sequencing was Alison Cloutier, a co-author of the new paper, who formerly worked with Baker and later became a postdoctoral researcher in Edwards’ lab at Harvard, which inherited the data/research.

Reconstructing the genome of a long-extinct bird fills in a new branch of the avian family tree, opening doors to study avian evolution, or even someday, to possibly resurrect these species through de-extinction technologies.

“To me, this work is all about fleshing out the natural history of this amazing species,” Edwards said.

-Note: This news release was originally published on the Harvard University website. As it has been republished, it may deviate from our style guide.

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