a vial of red fluid being placed in a cryogen dewar emphasizing the importance of cryogenic safey

Safe Cryogenics Practices and Procedures for Laboratory Employees

Many of the safety precautions observed for compressed gases also apply to cryogenic liquids.

Written byTrevor Henderson, PhD
Updated | 4 min read
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Understanding Cryogenic Liquids

Cryogenic liquids are substances with boiling points below -73ºC (-100ºF). Commonly used in laboratories, liquid nitrogen, liquid oxygen, and carbon dioxide play a vital role in many scientific and industrial applications. However, these materials pose serious hazards, including fire risks, explosions, embrittlement of materials, pressure buildup in containers, frostbite, and asphyxiation. Each of these dangers can have severe consequences if proper precautions are not taken.

Laboratory workers must adopt comprehensive safety measures to mitigate these risks. This involves understanding the unique properties of cryogenic liquids, implementing rigorous handling procedures, and maintaining a secure and well-equipped work environment to prevent accidents and ensure the safety of all personnel.

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Key Hazards of Cryogenic Liquids

Cryogenic liquids pose unique risks due to their extreme properties. Below are two primary hazards:

1. Extremely Low Temperatures

The extremely low temperatures of cryogenic liquids can freeze human tissue upon contact, leading to cold burns and frostbite. Materials like carbon steel, plastics, and rubber become brittle and may fracture under stress at these temperatures. Proper material selection for storage and handling equipment is crucial to minimize risks.

2. Vaporization and Pressure Buildup

All cryogenic liquids vaporize, producing large volumes of gas. For instance:

  • Liquid nitrogen expands 696 times when vaporized.
  • Liquid argon’s expansion ratio is 847:1.
  • Liquid oxygen expands 862:1.
  • Liquid hydrogen expands 851:1.

If vaporization occurs in a sealed container, the resulting pressure can cause the vessel to rupture. To prevent such incidents, pressurized cryogenic containers are equipped with multiple pressure relief devices.

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Moreover, the vaporization of cryogenic liquids in confined spaces may lead to asphyxiation or create flammable mixtures, especially with hydrogen or oxygen-rich atmospheres.


Safe Handling of Cryogenic Liquids

Proper handling procedures are essential to mitigate risks associated with cryogenic liquids, as these substances pose unique dangers such as frostbite, pressure buildup, and asphyxiation. By understanding the hazards and implementing safe handling practices, laboratory professionals can protect themselves and maintain a secure work environment. Follow these guidelines to ensure safety when working with cryogenic materials:

  • Avoid Direct Contact: Cryogenic liquids can cause severe burns and frostbite. Even brief exposure can damage sensitive tissues, such as the eyes. Always handle these liquids with care.
  • Minimize Boiling and Splashing: When filling warm containers or inserting objects, perform these tasks slowly to reduce boiling and splashing. Use tongs to withdraw items immersed in cryogenic liquids.
  • Never Touch Uninsulated Surfaces: Avoid touching uninsulated pipes, vessels, or other materials containing cryogenic liquids, as skin can adhere to the cold surface, causing serious injury.
  • Use Appropriate Tools: Use wooden or rubber tongs to remove small items from cryogenic liquid baths. Cryogenic gloves are designed for splash protection only and should never be immersed in cryogenic liquids.
  • Limit Filling: Fill cylinders and dewars to no more than 80% of their capacity to prevent excessive pressure buildup during warming.
  • Monitor Cold Baths: Regularly check cryogenic baths to ensure they are not obstructed by frozen materials.

Personal Protective Equipment (PPE) for Cryogenics

Wearing the right protective gear is crucial when handling cryogenic materials, as these substances pose significant risks, including frostbite, cold burns, and exposure to harmful vapors. Proper protective equipment acts as a barrier against these dangers, ensuring both personal safety and compliance with laboratory safety standards.

  • Eye and Face Protection: Use face shields along with safety glasses or chemical splash goggles to protect your eyes and face from splashes and vapor.
  • Cryogenic Gloves: Wear loose-fitting, dry, insulated gloves for handling objects that come into contact with cryogenic liquids and vapor. These gloves offer splash protection but are not suitable for immersion.
  • Proper Clothing: Ensure that trousers are worn over boots or work shoes to prevent liquid entry. Always wear dry, insulated clothing to avoid cold burns.

Cooling Baths and Dry Ice Safety

Cooling baths and dry ice are frequently used in laboratory procedures, but they come with their own set of risks. Proper precautions must be taken to prevent accidents, such as explosions, frostbite, or suffocation caused by the buildup of gases like carbon dioxide. Understanding the potential hazards and implementing safe practices ensures that cooling baths and dry ice can be used effectively without compromising safety.

  • Avoid Flammable Mixtures: Do not use liquid nitrogen or liquid air to cool flammable mixtures in the presence of air, as oxygen condensation can lead to explosion hazards.
  • Dry Ice Precautions: Use insulated gloves and a face shield when handling dry ice. Add dry ice slowly to cooling baths to prevent foam-over. Avoid lowering your head into dry ice chests, as carbon dioxide buildup can cause suffocation.

Safe Use of Liquid Nitrogen Cooled Traps

Traps cooled with liquid nitrogen that are open to the atmosphere can rapidly condense liquid air. If the system is closed, pressure can build to dangerous levels, potentially shattering glass equipment. To prevent accidents:

  • Use only sealed or evacuated equipment for liquid nitrogen-cooled traps.
  • Regularly inspect traps to ensure they function correctly and safely.


Frequently Asked Questions (FAQs)

1. What are the main risks associated with cryogenic liquids?

The primary risks include frostbite, cold burns, asphyxiation due to vaporization, pressure buildup in sealed containers, and the potential for explosions when handling flammable cryogenic materials like hydrogen.

2. How can I safely handle objects immersed in cryogenic liquids?

Always use appropriate tools such as wooden or rubber tongs to handle objects. Wear insulated cryogenic gloves designed for splash protection and ensure that no direct contact with cryogenic liquids occurs.

3. Why is it important to limit the filling of cryogenic containers to 80% capacity?

Filling cryogenic containers beyond 80% capacity increases the risk of excessive pressure buildup as the liquid warms and expands. This can lead to ruptures or dangerous leaks, compromising safety in the lab.

Conclusion: Ensuring Cryogenic Safety

Handling cryogenic liquids requires strict adherence to safety protocols to prevent serious injuries and equipment damage. By understanding the hazards, wearing appropriate protective gear, and following safe handling and storage practices, laboratory professionals can ensure a safe working environment. Regular training and equipment maintenance are vital to reducing risks and maintaining safety standards.


About the Author

  • Trevor Henderson headshot

    Trevor Henderson BSc (HK), MSc, PhD (c), has more than two decades of experience in the fields of scientific and technical writing, editing, and creative content creation. With academic training in the areas of human biology, physical anthropology, and community health, he has a broad skill set of both laboratory and analytical skills. Since 2013, he has been working with LabX Media Group developing content solutions that engage and inform scientists and laboratorians. He can be reached at thenderson@labmanager.com.

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