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The Ins and Outs of Digital PCR

The Ins and Outs of Digital PCR

Absolute quantification of target nucleic acids using partitioning

by Lab Manager

Digital polymerase chain reaction (dPCR) enables absolute quantification of target nucleic acids in a sample. The sample is partitioned into many individual reactions, each containing only a few target sequences. Following PCR amplification, amplified target sequences are detected by fluorescence and the ratio of positive partitions to total partitions is used to determine the concentration of the target sequence in the sample. Quantification is based on the random distribution of molecules in multiple partitions that follows a Poisson distribution. 

In this infographic you'll learn more about:

  • dPCR advantages
  • How it Works: specifics of dPCR
  • Valuable information about partitioning technologies

Download the full infographic, compliments of Lab Manager.