Live Air Date: Thursday, February 27, 2014
Raman microscopy is routinely used for characterization and identification of material, but the need for this molecular imaging and analysis technique has become increasingly important in biology. Advances in imaging techniques such as StreamLine™ Plus, combined with chemometric data analysis capabilities, make it possible to gain an understanding of chemical changes within biological materials. Raman’s ability to probe the chemical and molecular structure of biological materials is obtained directly without the need for dyes or markers. Renishaw’s inVia can be utilized to generate chemical images for cells, tissues, and bone with high spatial resolution. It has been employed for cancer diagnosis, stem cell differentiation, protein structure analysis, diagnostics, and bacterial identification.
As an attendee, you will learn more about:
- A Raman technique used to identify cancer cells from healthy cells
- A Raman technique used to analyze lipid based drug discovery
- Set up of an experiment using label free detection (no fluorescent dyes, colorimetric stains or labeled antibodies needed)
Dr. Frederick Coffman is the Associate Director of the Center for Biophysical Pathology at Rutgers New Jersey Medical School. He has a longstanding interest in cancer research, particularly in defining differences between cancer cells and their normal counterparts for applications in diagnosis, prognosis and therapy. As the biophysical properties of cells are both reflective of gene expression patterns and predictive of abilities to migrate and to interact with cells and tissues, a major research focus involvesmeasuring biophysical properties such as dynamic mass rearrangement, impedance, and laser Raman spectra of various tumor and normal cells to define tissue-specific and stage-specific signatures of highly malignant cancer cells. Dr. Coffman has also studied the chitinase family protein YKL-40 which appears to function as a survival factor in cancer cells, the regulation of DNA replication initiation in leukemia cells, and the synergistic enhancement of tumor cell killing by TNF by specific classes of DNA topoisomerase II inhibitors. He has lectured on diverse subjects from DNA structure to genetics to cancer in the graduate, medical, dental, and pre-professional schools, and is currently course director of the graduate core class in biomedical sciences.