Bio-Rad Introduces EveryBlot Blocking Buffer that Offers 5-Minute Blocking Time and Greater Sensitivity for Western Blots

EveryBlot offers superior sensitivity and low background with both chemiluminescent and fluorescence detection across a wide range of targets

Written byBio-Rad Laboratories
| 2 min read
Register for free to listen to this article
Listen with Speechify
0:00
2:00

HERCULES, CA — November 11, 2019 — Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) today announced the launch of EveryBlot Blocking Buffer, a western blot blocking reagent that offers five-minute blocking time and produces higher signal and lower background levels resulting in greater sensitivity for western blots compared to other available blocking buffers.

Bio-Rad EveryBlot Blocking BufferEveryBlot Blocking Buffer provides five-minute blocking and maximum sensitivity for western blots, regardless of the detection method.Credit: Bio-Rad LaboratoriesWhen attempting to detect a new protein target, researchers often optimize several variables including the use of specific blocking buffers (reagents) when performing western blots. For example, many blocking buffers are optimized for specific detection methods such as chemiluminescence or fluorescence detection and some buffers may not be compatible for detection of some targets. EveryBlot offers superior sensitivity and low background with both chemiluminescent and fluorescence detection across a wide range of targets. In addition, available blocking buffers require blocking times of up to an hour, while EveryBlot Blocking Buffer completes the blocking step in five minutes.

"EveryBlot works with all detection methods and has been tested for compatibility across a wide range of protein targets, which should help eliminate the worry that comes when attempting to detect a new protein on a western blot," said Paul Liu, Bio-Rad product manager, western blotting.

Interested in life sciences?

Subscribe to our free Life Sciences Newsletter.

Is the form not loading? If you use an ad blocker or browser privacy features, try turning them off and refresh the page.

Key Benefits of EveryBlot Buffer

  • Offers five-minute blocking time, shortening the duration of the immunodetection step
  • Offers higher signal and lower background levels therefore greater sensitivity than blots blocked with other available buffers
  • Prevents non-specific binding to increase sensitivity
  • Compatible with all detection methods as well as for detection of phosphorylated targets, which eliminates the need for optimization

To learn more about EveryBlot Blocking Buffer, please visit www.bio-rad.com/SeeEveryBlot

About Bio-Rad

Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) is a global leader in developing, manufacturing, and marketing a broad range of innovative products for the life science research and clinical diagnostic markets. With a focus on quality and customer service for over 65 years, our products advance the discovery process and improve healthcare. Our customers are university and research institutions, hospitals, public health and commercial laboratories, biotechnology, pharmaceutical, as well as applied laboratories that include food safety and environmental quality. Founded in 1952, Bio-Rad is based in Hercules, California, and has a global network of operations with more than 8,000 employees worldwide. Bio-Rad had revenues exceeding $2.2 billion in 2018. For more information, please visit www.bio-rad.com.

Loading Next Article...
Loading Next Article...

CURRENT ISSUE - April 2025

Sustainable Laboratory Practices

Certifications and strategies for going green

Lab Manager April 2025 Cover Image