New ChemiDoc Go imaging system on a white background

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Bio-Rad Laboratories

The ChemiDoc&trade; Go Imaging System can help improve efficiency in the lab

Introducing a New Imaging System for Enhanced Lab Efficiency

Product News

Western blot comparisons and a line chart

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Researchers must follow best practices for data normalization

Maintaining Data Integrity for Western Blotting Experiments

Article

Why Validating Your Antibodies is Crucial for Success

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557032

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Recommended methods and controls to determine if an antibody is recognizing its intended target

Bethyl Laboratories/Fortis Life Sciences

When it comes to research use, there are no standard guidelines in place for manufacturing, validating, and using antibodies

Infographic

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New Magnetic Bead Platform with Environmentally Friendly Design

Thermo Fisher Scientific

The DynaGreen&trade; Magnetic Beads can help labs adopt more sustainable practices

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photo representation of the western blot workflow

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a scientist using western blotting techniques in the lab

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Cytiva UK Limited

Cytiva

A handbook for everyone, from beginners to experts

Are You Up to Date on Western Blot Workflows?

Whitepaper

Application Note Cover: ImageQuant™ 800 imager: using dyes across the spectrum

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A latex-gloved hand squeezes liquid from a small bottle onto a testing disc

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Fig 2. The ImageQuant™ 800 imaging system comes equipped with touchscreen, two tray positions, and an easy-access filter door.

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Fig 4. Fluorescence emission spectra of CPNs™ from Stream Bio’s (spanning 420 nm to 1000 nm). CPNs™ fluorescence at specific wavelengths is due to the chemical composition. The excitation and emission characteristics of the CPNs™ are tailored by selecting specific conjugated polymers for the core.

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Fig 6. Multiple replicates show consistent strong signal from CPNs™ with higher sensitivity than Europium.

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Fig 7. Nitrocellulose membrane spotted with 50 ng of full-length spike protein, S1, and RBD of the virus SARS-CoV2. The image was captured using the UV epi (365 nm) and Cy™2 (525BP20) filter for the CPN510B signal.

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Fig 5. CPN610 or europium was used as detection agents by linking to anti-hCG detection antibodies. The strips were imaged using the ImageQuant™ 800 and the signal from the test and control lines analyzed to give a mean pixel intensity for each line. The image was captured using the Blue epi (460 nm) and Cy™3 UV filter for the CPN610 signal and UV epi (365 nm) and Cy™3 UV filter for the europium signal. White grayscale was used to indicate fluorescence signal, i.e., a high signal is shown as white in the image.

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Fig 9. Lateral flow strips were run using a range of infrared CPNs™ as detection agents and the strips were imaged using four different filter sets on the ImageQuant™ 800. White grayscale was used to indicate fluorescence signal, i.e., a high signal is shown as white in the image.

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Fig 10. Mouse-anti-CD44 (500 ng 1 ?g) was run on a PAGE gel and transferred to a nitrocellulose membrane. The membrane was probed with CPN840 linked to anti-mouse IgG which selectively bound, giving a bright signal at the expected size (150 kDa). The image was captured using the 660 nm IR short epi and 46 nm IR long (836BP) filter for the CPN840 signal. White grayscale was used to indicate fluorescence signal, i.e., a high signal is shown as white in the image.

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Conjugated polymer nanoparticles and optimized imaging systems boost lateral flow assay development with new capabilities

Increasing Photostability and Target Specificity for Lateral Flow Assays and Western Blots

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Download the free ebook to learn how you can save time and deliver high-resolution, high-quality, and high-confidence life science images with western blotting.

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Work more efficiently, freeing valuable resources to drive your research forward, faster 

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Selecting the right biomolecular imager for your workflows increases efficiency and data quality

How to Improve Imaging Workflows for Western Blots

Resource Guide

Women scientist doing researches on coronavirus.

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Bio-Techne

Bringing the western blot into the 21st century

A New Frontier in Fluorescence Detection Sensitivity

An immunoprecipitation with antibodies then immunoblotted to show antibody validation

Independent antibody and complementary assays for validation

Are Your Antibodies Valid?

Internationally recognized validation standards ensure antibodies are specific, selective, sensitive, and reproducible

Product Focus

Track Western Blot Workflows

Traceability in Western blot imaging: Considerations for GxP

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AI & Automation

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Lab Manager

Outsourcing for Quality, Efficiency, and Flexibility

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NuAire

The Key Decisions That Define Biosafety Cabinet Performance&nbsp;

Water Purification Resource Guide Thumbnail

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MilliporeSigma

The Role of Water in Laboratory Accuracy

Headshot of Mark Sykes, head of science for Fapas Proficiency Testing and a principal scientist at Fera Science Ltd

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Scientist performing quality control test on a food product as part of proficiency testing

Western Blot