image of a older male lab technician working at a brightly lit fume hood emphasing the importance of replacing fume hood lighting

Replacing fluorescent light tubes in chemical fume hoods requires careful preparation and adherence to safety protocols.

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Changing Fluorescent Light Tubes in Chemical Fume Hoods

Routine service of laboratory fume hoods includes the periodic changing of burned-out fluorescent light tubes. 

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Routine maintenance of chemical fume hoods often involves changing burned-out fluorescent light tubes. Although these hoods are designed to exhaust hazardous materials, small amounts of chemical residue can remain, necessitating special precautions. Proper preparation and adherence to safety protocols are critical to protect both personnel and equipment. Below are comprehensive guidelines to ensure safe and efficient light tube replacement in fume hoods.

Steps to Follow to Replace Fluorescent Light Tubes in a Fume Hood


Preparation for Work

1. Hazard Determination
Certain fume hoods may contain residues of highly hazardous substances, such as perchloric acid, requiring special precautions. Evaluate the specific risks associated with the hood before proceeding.

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2. Preparation of the Hood Work Area

  • For Bulbs Changed from Above the Hood
    Ensure all hazardous materials inside the hood are capped or secured to prevent accidental release. Active experiments should be paused, and highly hazardous substances should be removed entirely. Clear any obstructions near the hood to allow safe placement of a ladder.
  • For Bulbs Changed from Inside the Hood
    Remove all hazardous materials, equipment, and experimental apparatus from the hood. As with above-hood servicing, ensure the surrounding area is clear to facilitate safe ladder placement.

3. Cleaning and Decontamination

  • Above-Hood Servicing
    Clean and decontaminate the sash and any areas of the hood that may come into contact during maintenance.
  • Inside-Hood Servicing
    Decontaminate all surfaces within the hood before beginning work to minimize exposure to residual chemicals.

4. Special Procedures

  • Radioisotope Hoods
    Survey the hood for contamination before replacing light tubes. Clean surfaces to meet radiological contamination limits, and shield any fixed contamination appropriately.
  • Hoods Handling Biological Materials
    Wipe down all surfaces with a suitable disinfectant to eliminate biological hazards.
  • Special Hazard Hoods
    Follow additional safety protocols for hoods that have handled unique hazardous materials, such as perchloric acid.

5. Preparation of the Work Area—Additional Considerations
Ensure the ladder is in good condition and appropriately sized. Turn off the power to the light source before beginning maintenance.


Personal Protective Equipment (PPE)

Wear the recommended protective apparel, including:

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  • Latex or nitrile gloves to protect hands from contaminants.
  • Safety glasses with side shields to guard against debris and splashes.
  • A lab coat to shield clothing from dust and potential chemical exposure.

If the work generates dust, consider wearing a dust mask or respirator as appropriate.


Performing the Work

1. Ergonomics
Be mindful of your posture to avoid musculoskeletal strain. Adjust the ladder or work surface to minimize awkward or forceful movements. Take short breaks if the task requires prolonged periods in uncomfortable positions.

2. Lighting
Use a flashlight to illuminate dark areas, especially if pipes or equipment cast shadows over the work area. Proper lighting reduces the likelihood of accidents.


Completion of Activities

1. Clean Up the Work Site
After replacing the fluorescent tubes, clean the area thoroughly using wet cleaning methods. Collect and clean any tarps or drop cloths used during the process.

2. Disposal of Spent Tubes
Fluorescent light tubes contain mercury and must be treated as Universal Waste. Place spent tubes in designated disposal containers to prevent environmental contamination and comply with regulatory requirements.

3. Hygiene
Wash hands thoroughly with soap and water after completing the task to remove any residual contaminants.


Frequently Asked Questions

1. Why is it important to clean and decontaminate the hood before changing light tubes?

Cleaning and decontaminating the hood ensures that any chemical residues or hazardous materials are removed, reducing the risk of exposure during maintenance.

2. What PPE should I wear while changing fluorescent light tubes in a fume hood?

You should wear latex or nitrile gloves, safety glasses with side shields, and a lab coat. If dust is present, consider using a dust mask or respirator for added protection.

3. How should I dispose of spent fluorescent light tubes?

Fluorescent light tubes contain mercury and must be disposed of as Universal Waste. Place them in designated containers to comply with safety and environmental regulations.


Conclusion: Ensuring Safe Fume Hood Maintenance

Replacing fluorescent light tubes in chemical fume hoods requires careful preparation and adherence to safety protocols. By evaluating hazards, using appropriate PPE, and following proper cleaning and disposal procedures, you can protect both personnel and the laboratory environment. Routine maintenance conducted safely ensures that fume hoods remain effective in exhausting hazardous materials and maintaining a safe workspace.


About the Author

  • Trevor Henderson headshot

    Trevor Henderson, PhD

    Trevor Henderson BSc (HK), MSc, PhD (c), is the creative services director at Lab Manager.  He has more than two decades of experience in the fields of scientific and technical writing, editing, and creative content creation. With academic training in the areas of human biology, physical anthropology, and community health, he has a broad skill set of both laboratory and analytical skills. Since 2013, he has been working with LabX Media Group developing content solutions that engage and inform scientists and laboratorians. He can be reached at thenderson@labmanager.com.

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