Chemists Develop Faster, More Efficient Protein Labeling

North Carolina State University researchers have created specially engineered mammalian cells to provide a new “chemical handle” which will enable researchers to label proteins of interest more efficiently.

| 2 min read
Register for free to listen to this article
Listen with Speechify
0:00
2:00

North Carolina State University researchers have created specially engineered mammalian cells to provide a new “chemical handle” which will enable researchers to label proteins of interest more efficiently, without disrupting the normal function of the proteins themselves or the cells in which they are found.

Protein labeling is used by researchers in a variety of fields to help them understand how these important molecules affect the normal functioning of cells. Currently, proteins are labeled for study simply by fusing them to other fluorescent proteins, which allows researchers to use microscopy to track their movements through a cell. This approach has several drawbacks, however, not least being that the fluorescent proteins are often large enough to affect the function of the protein of interest.

Want to stay up to date on the latest lab management news?

Subscribe to our free Lab Manager Monitor Newsletter.

Is the form not loading? If you use an ad blocker or browser privacy features, try turning them off and refresh the page.

Dr. Alex Deiters, associate professor of chemistry, along with colleague Dr. Jason Chin of the Laboratory of Molecular Biology at the Medical Research Council in Cambridge, U.K., have developed a way to attach a fluorophore – a fluorescent molecule about 20 times smaller than the fluorescent proteins currently in use – to a protein that is expressed in a mammalian cell.

Deiters and Chin developed a special 21st amino acid that they added to cells that were specially engineered to incorporate this amino acid into the protein they wanted to study (there are normally only 20 amino acids). This 21st amino acid has a “chemical handle” that only reacts with a specifically designed fluorophore, but not any cellular components. According to Deiters, “The reaction between the modified protein and the fluorophore is extremely fast, high yielding, and generates a stable link between both reaction partners. This novel methodology enables future cell biological studies that were previously not possible.”

The research appears in the Feb. 5 issue of Nature Chemistry.

“We found that our approach gave us a higher yield of labeled proteins and that the binding reaction was 50 times faster than with current methods,” Deiters says. “Additionally, it took less reagent to complete the reaction, so overall we have a faster, more efficient method for protein labeling, and less chance of interfering with the normal function of the proteins and cells being studied.”

The research was funded by the National Institutes of Health and the National Science Foundation. The Department of Chemistry is part of NC State’s College of Physical and Mathematical Sciences.

Related Topics

Loading Next Article...
Loading Next Article...

CURRENT ISSUE - January/February 2025

Energizing Leadership in Action

The science-backed behaviors that help leaders inspire thriving teams and organizations

Lab Manager January/February 2025 Cover Image