Scientist using a Biological Safety Cabinet for research

Ensuring Proper Use and Maintenance of Biological Safety Cabinets in Laboratories

From certification to daily cleaning, consistent attention to biological safety cabinet practices strengthens both lab safety and research integrity

Written byDavid Phillips
| 2 min read
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Research involving potentially biohazardous materials is essential for addressing some of the most pressing public health concerns. Laboratories engaged in this research are equipped with specialized devices that not only advance investigations but also protect researchers. Proper usage and maintenance of these devices are crucial for their optimal functionality. One of the most common devices for protecting against biohazards and reducing contamination is the biological safety cabinet (BSC).

The importance of proper BSC certification and expert oversight

BSCs must meet stringent standards that require regular performance certification and adjustment, at least annually. This certification process allows the cabinet's operation to be reviewed by an expert with specialized knowledge of its functionality. During my tenure as a certifier, I encountered various non-standard practices, such as signs warning “Do not close the window! It will jam!” and tape over switches, which led to valuable conversations. Sometimes, repairs were necessary, but often, users simply needed guidance on proper operation. These interactions invariably resulted in improved cabinet performance for the users.

Why proper use matters as much as maintenance

Beyond testing and certification, most BSCs do not require traditional preventive maintenance tasks such as lubricating moving parts, replacing components on a schedule, or rebuilding assemblies. However, proper usage of the BSC is equally important. Using a BSC effectively can be challenging, akin to playing pin-the-tail-on-the-donkey but only looking to see how we did much later. Effective use involves working with airflows that are barely perceptible to control particles that are invisible, and this must be done multiple times a day, most days of the week, for years. Therefore, habits, routine, and consistency are essential.

Best practices for surface decontamination

BSC airflows control the airborne contamination, while decontamination of surfaces controls contact contamination. BSCs are designed to facilitate easy surface decontamination, but routine cleaning must be supported to move beyond mere intentions of improvement. Here are some recommendations:

  • Provide a designated place for surface decontamination materials next to the cabinet being cleaned. It is difficult to do a methodical wipe down when your hands are full.
  • Minimize or eliminate equipment and supplies left inside the cabinet. Although it may be inconvenient to remove and replace items, it is also challenging to clean around them. A possible solution is to remove everything for cleaning at the start and end of the day, with periodic cleaning around equipment during the day.
  • Recognize that this is not about skill or intelligence but about adhering to basic protocols. Just as wearing a seatbelt does not reflect on one's driving skills, routine cleaning practices are necessary for everyone, regardless of expertise.

A beneficial side effect of thorough cleaning, including wiping down supplies before placing them into the cabinet, is the heightened awareness of how easily contamination can occur.

About the Author

  • David Phillips has been involved with biological safety cabinets (BSCs) for over 40 years. He was a BSC field certifier until 2007, when he joined Thermo Fisher Scientific’s BSC product team. He is on the NSF Joint Committee for NSF/ANSI 49 and has been an NSF Accredited BSC Field Certifier under the Enhanced program for over 24 years. He has a doctorate in management from the University of Maryland. His key areas of interest are improving BSC sustainability and effective access to BSCs globally.

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