Technician reviews a glass of craft beer as part of the scientific brewing process

The Science of Craft Beer: How UV-Vis Spectrophotometry Enhances Brewing Quality

Discover how UV-Vis spectrophotometry enhances craft beer quality by optimizing color, bitterness, and consistency in modern brewing.

Written byTrevor Henderson, PhD
Updated | 3 min read
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The craft beer industry has exploded in popularity over the last few decades, with hundreds of new breweries emerging annually across the United States. While traditional light lagers from major breweries like Coors and Budweiser once dominated the market, today's beer consumers demand stronger, hoppier, and more diverse brews.

Why UV-Vis Spectrophotometry is Essential for Brewing

UV-Vis spectrophotometry is widely used in brewing because of its ability to provide rapid, precise, and repeatable measurements. This technology enables brewers to:

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  • Monitor beer quality at multiple stages of production
  • Ensure consistency across different batches
  • Analyze key components that impact flavor, color, and stability
  • Optimize ingredient usage to enhance efficiency

According to Rick Blankemeyer, quality assurance analyst at Stone Brewing Company (Escondido, CA), UV-Vis spectroscopy plays a crucial role in maintaining product consistency:

“We use the UV-spec to monitor multiple aspects of the brewing process to ensure we are producing consistent beer across our many styles.” 


What Can UV-Vis Spectroscopy Measure in Beer?

UV-Vis spectrophotometry is a versatile analytical tool that can evaluate a range of chemical and physical properties in beer. Here are some of the most important brewing parameters it can assess:

1. Beer Color Analysis

  • The Standard Reference Method (SRM) is used to determine beer color using UV-Vis spectrophotometry at 430 nm.
  • Helps brewers maintain color consistency across batches.
  • Essential for ensuring that light lagers, IPAs, and stouts have the expected appearance.

🔹 Example: A craft brewery making an amber ale must ensure the correct reddish-gold hue in every batch. UV-Vis spectroscopy provides objective color measurements to maintain uniformity.

2. Protein Content and Beer Haze

  • Proteins play a crucial role in head retention and mouthfeel.
  • UV-Vis helps identify excessive protein content, which can cause haze formation.
  • Brewers can adjust filtration and processing steps to improve clarity.

🔹 Example: A wheat beer is expected to have some haze, but an unintended cloudy appearance in a pale lager could indicate protein instability.

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3. Polyphenols and Bitterness (Hops Content)

  • Bitterness units (IBUs) are measured using UV-Vis at 275 nm to quantify hop compounds.
  • Polyphenols from malt and hops can impact astringency and stability.
  • Allows brewers to fine-tune hop additions for optimal flavor balance.

🔹 Example: A double IPA needs precise hop bitterness control to achieve a balance between malt sweetness and hop intensity.

4. Ethanol Concentration and Fermentation Efficiency

  • UV-Vis can quantify ethanol levels during fermentation.
  • Helps brewers ensure desired alcohol content without the need for time-consuming distillation methods.
  • Ensures compliance with labeling regulations on ABV (Alcohol by Volume).

🔹 Example: A stout marketed as 7.5% ABV must be tested to confirm its actual alcohol percentage meets regulations and consumer expectations.

5. Iodine Test for Starch Conversion

  • Starch-to-sugar conversion efficiency is monitored using iodine-based UV-Vis tests.
  • Ensures mashing is fully converting starches into fermentable sugars.

🔹 Example: If iodine testing shows remaining starch, the mash needs further enzymatic breakdown to optimize fermentation efficiency.

6. Sulfites and Antioxidant Levels

  • Sulfites are used as preservatives to extend shelf life.
  • UV-Vis spectroscopy can detect sulfite levels to prevent excessive use.
  • Also helps in monitoring oxidation processes, which can affect beer freshness.

🔹 Example: A craft brewery bottling an IPA for long-distance distribution needs to ensure it doesn’t oxidize too quickly, affecting flavor and aroma.

7. Total Carbohydrate Content

  • Carbohydrates influence body, sweetness, and caloric content.
  • UV-Vis tests allow brewers to fine-tune sugar profiles in different beer styles.

🔹 Example: A low-carb beer requires careful monitoring of residual sugar levels to meet market trends and consumer preferences.


The Future of UV-Vis Spectrophotometry in Craft Brewing

As craft breweries continue to push innovation, quality control measures like UV-Vis spectrophotometry will become even more critical. Future trends include:

  • Automated UV-Vis systems for real-time monitoring and quality control.
  • Integration of AI and machine learning for predictive brewing adjustments.
  • Expanded use in experimental brewing, including alternative grains and fermentation techniques.

Final Thoughts: Why UV-Vis is a Must-Have for Craft Brewers

For craft breweries looking to enhance quality, maintain consistency, and optimize efficiency, UV-Vis spectrophotometry is a game-changer. With its ability to analyze multiple brewing parameters faster and more accurately than traditional methods, this technology is an invaluable asset for brewers striving to meet consumer demand for diverse and high-quality beer.

Key Takeaways:

  • UV-Vis spectrophotometry ensures consistent beer quality and appearance.
  • It helps brewers optimize color, bitterness, protein stability, and fermentation efficiency.
  • UV-Vis is an essential tool for modern breweries looking to improve efficiency and product consistency.

Would you like to learn more about how UV-Vis technology can elevate your craft brewing process?

➡️ Download Lab Manager’s On-Demand Webinar: “An Overview of UV-Vis Spectrophotometry in the Craft Brewing Industry.”


About the Author

  • Trevor Henderson headshot

    Trevor Henderson BSc (HK), MSc, PhD (c), has more than two decades of experience in the fields of scientific and technical writing, editing, and creative content creation. With academic training in the areas of human biology, physical anthropology, and community health, he has a broad skill set of both laboratory and analytical skills. Since 2013, he has been working with LabX Media Group developing content solutions that engage and inform scientists and laboratorians. He can be reached at thenderson@labmanager.com.

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