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Transferring Methods from HPLC to UHPLC

How to optimize your liquid chromatography workflows for lower long-term costs

PerkinElmer LC 300 platform

Whether your application requires the ruggedness of HPLC or the precision and power of UHPLC, the PerkinElmer LC 300 platform paired with SimplicityChrom™ CDS software and a broad LC column portfolio redefines your liquid chromatography experience.

by PerkinElmer
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Q: What are the advantages of transferring my liquid chromatography (LC) methods from high performance liquid chromatography (HPLC) to ultra-high performance liquid chromatography (UHPLC)?

LC supports a wide range of applications and is ideal for laboratories aiming to increase throughput. It also offers good performance and analytical flexibility. HPLC is one of the most widely used analytical techniques, however, as needs change many laboratories are transitioning their HPLC methods to UHPLC methods. Not all analytical methods are good candidates for method transfer, and there are several factors to consider ensuring a successful migration to UHPLC.

A:Converting methods from HPLC to UHPLC offers substantial benefits in high-throughput settings. 

UHPLC enables rapid analytical separations and can increase productivity. It also offers better peak resolution which can result in more confident analyte identification and quantification. Since UHPLC requires less solvent, it can also reduce long-term costs and environmental impact. When considering conversion of a method from HPLC to UHPLC, several factors should be reviewed to ensure the new method offers consistent data quality and efficiency. The following factors can help identify analyses that are good candidates for migration to UHPLC:

• Longer analytical run times, typically ≥ 20 minutes

• Uses reverse phase columns

• Requires separation and quantification of many analytes

• Samples are filtered or relatively clean

• Anticipating demand for increased throughput

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