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Vector Laboratories Introduces First Effective Reagent for Autofluorescence Quenching

New quenching reagent enables researchers to identify specific markers that cannot be seen without removing the background noise of autofluorescenc

by Vector Laboratories
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TrueVIEW™ Autofluorescence Quenching KitAdjacent human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor® Duet kit (DK-8818). Note significant reduction of auto fluorescence in the treated section (right) with retention of well-defined, specific signal in both red and green channels.BURLINGAME, Calif. (November 13, 2017) – Vector Laboratories, a leader in the development and manufacture of labeling and detection reagents for biomedical research, introduces the TrueVIEW™ Autofluorescence Quenching Kit, offering the first effective technique for removing unwanted fluorescence in formalin-fixed paraffin embedded (FFPE) tissue samples, blood cells, and structural elements such as collagen and elastin. Autofluorescence often impairs or prevents the use of immunofluorescence assays when the specific stained antigen cannot be distinguished from the interfering background signal. This is particularly problematic with tissue samples preserved using the FFPE method. TrueVIEW specifically targets background autofluorescence, and retains the intended specific fluorescent staining. TrueVIEW is effective across the spectral range from blue to far red and is compatible with commonly used fluorophores. This new quenching reagent enables researchers to identify specific markers that cannot be seen without removing the background noise of autofluorescence. 

“Other approaches to removing tissue autofluorescence have been ineffective, laborious, and can even reduce the immunofluorescence signal of the sample. With our new TrueVIEW Kit, we improve the signal-to-noise ratio,” explains Craig Pow, PhD, director of technical services for Vector Laboratories. “We anticipate that this new reagent will benefit researchers using immunofluorescence with FFPE samples, particularly those studying spleen, pancreas, or kidney tissue. We also expect that the ability to remove background noise will expand the use of immunofluorescence in archived cancer, histopathology, neurobiology, and cell biology research specimens.” 

Applying TrueVIEW to sections is fast, requiring just five minutes for incubation. Ethanol steps and dilution are not required. The Vector TrueVIEW kit includes VECTASHIELD HardSet Antifade mounting media and contains enough reagent for between 100 and 150 tissue sections. 

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About Vector Laboratories

Vector Laboratories develops, manufactures and markets labeling and detection reagents for immunohistochemistry, immunofluorescence, DNA/RNA, and glycobiology. The company serves customers in disease and therapeutics research with tools that help them precisely visualize and study tissues and cells. A pioneer in a number of innovative technologies, Vector Laboratories has been providing a continuous stream of reliable tools to its customers for more than 40 years. With headquarters in Burlingame, California and a subsidiary in Peterborough, U.K., the company maintains a network of more than 40 distributors across the globe. A privately held company, Vector Laboratories was acquired by Maravai LifeSciences in 2016.