In HPLC columns, reverse-phase and normal-phase chromatography separation methods are based on polarity. Reverse phase columns separate analytes based on their hydrophobicity, with the more hydrophobic compounds being retained longer on the column.

Column technology has been a rich area for HPLC R&D. As reasonably expensive consumables, columns are a significant factor in LC operating costs and performance— in other words, key value drivers.

HPLC systems are forced to their pressure limits under fast HPLC and high resolution HPLC conditions. System back pressure issues can arise from efforts to increase efficiency and resolution such as increasing flow rates and column lengths, as well as reducing particle size.

High-performance liquid chromatography (HPLC) columns are considered the “heart” of the instrument used to transport the analyte and the mobile phase and provide the environment in which separation is achieved. 

Abstract: The purification of alkaloids using RediSep Strong Cation Exchange (SCX) columns is described. Several alkaloids are used as models for those extracted from plant species. The alkaloids are separated from neutral and acidic compounds, and a

At the end of March, Lab Manager held a “Product Showcase” webinar on “New Chemistries and Improvements in Liquid Chromatography (LC) Columns” featuring a panel of experts from leading providers of LC columns and equipment.