Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, has developed an extensive quantitative real-time polymerase chain reaction (qPCR) user guide, which is available to download for free from www.idtdna.com.
RNA interference (RNAi) uses short strands of synthetic ribonucleic acid (RNA) to silence or “knock down” genes implicated in certain phenotypes—most commonly (but not limited to) diseases. The “interference” occurs when