An end user shares how her company uses mass spectrometry and how she went about selecting the MS systems in her facility in a brief Q&A.

This month Lab Manager poses four questions on GC usage, likes, and dislikes to a panel of five experts

Process GC involves the deployment of rugged, reliable gas chromatographs in demanding process environments. Where traditional sampling and analysis occurs off-site in analytical laboratories, process GC brings the “lab” to the production site, providing realtime product analysis.

The proverbial “ounce of prevention” goes a long way toward preventing serious GC downtime. Keeping up with routine maintenance is the secret to ensuring that scheduled maintenance downtime occurs on the lab’s terms, not by fickle fate.

Thermo Fisher’s Eric Phillips, describes the adoption of MS detectors in GC as a “technology shift” that began with single-quad MS as an alternative to standard GC detection modes.

Capillary columns have changed the face of GC since their introduction about 35 years ago. The most obvious change involves resolving power: up to 50,000 theoretical plates on a 30-m capillary versus 1500 on a six-foot packed column.

Despite steadily losing ground to high-performance liquid chromatography (HPLC) over the years, particularly for polar compounds, gas chromatography (GC) remains one of the more rapid and efficient chromatographic methods.

Choosing an in vitro model system that faithfully represents the natural physiology of the cell being studied is fundamental to understanding its in vivo function. Cultures of primary cell isolates retain their physiology and karyotype after isolation, but cultures may be difficult to prepare and are susceptible to contamination.

Basic research and clinical research labs have long relied on real-time PCR (qPCR) for its speed, sensitivity, specificity and ease-of-use. Common applications include gene expression analysis, mutation detection and identification of copy number variation to better understand inherited disorders, cancer and infectious disease.

Problem: In most UHPLC/ HPLC applications, the buildup of sample and mobile phase contaminants at the head of the chromatography column can cause detrimental effects.