Classical methods for protein quantitation rely on colorimetric assays, such as those involving protein-copper chelation (bicinchoninic acid (BCA) and Lowry assays) and dye-binding based detection (Bradford and “660 Assay”) or ultraviolet (UV) spectroscopy.

Protein affinity purification is typically carried out in a miniaturized format using spin columns or gravity flow methods. Both techniques have limitations.

Contributing editor Tanuja Koppal, Ph.D., talks to two scientists—one from a large pharmaceutical company and the other from a large academic institution—about their experiences with transitioning from paper to electronic lab notebooks (ELNS).

Whether your lab is growing or shrinking, training programs need to stay in sync with your changing requirements.

What lab managers need to now about new labeling, compliance dates, MSDS, training, and more.

Characterize chemical waste correctly to ensure safety & avoid fines and penalties.

Five rules for maintaining stakeholder support, managing costs and achieving goals.

Strong growth in healthcare spending, especially in emerging economies, is driving increased demand for medical testing, drug discovery, genomics, and proteomics, as well as the instruments used to carry out these processes.

Problem: A mainstay in life science research, western blotting is commonly used to assess relative protein expression differences, such as when investigating cellular signaling pathways. Accurate quantification requires careful attention to procedural details and normalization.

Since the early 1990s, biobanking has been evolving as a key resource, increasing the availability and use of biological material such as DNA, RNA, tissues and cells for biomedical research.

HPLC systems are forced to their pressure limits under fast HPLC and high resolution HPLC conditions. System back pressure issues can arise from efforts to increase efficiency and resolution such as increasing flow rates and column lengths, as well as reducing particle size.