Cross-contamination of human cell lines with other cell lines and/or misidentification of cell cultures is reported to be 18-36 percent. Find out one way to prevent contamination in your lab.

Dr. Amanda Capes-Davis, an independent cell culture consultant and founding manager of CellBank Australia, talks to contributing editor Tanuja Koppal, PhD, about the imperative need to establish best practices in a cell culture laboratory.

Choosing an in vitro model system that faithfully represents the natural physiology of the cell being studied is fundamental to understanding its in vivo function. Cultures of primary cell isolates retain their physiology and karyotype after isolation, but cultures may be difficult to prepare and are susceptible to contamination.

After applications and processes, workflow optimization is the primary consideration when setting up a cell culture lab. Workflow relates to how samples and cultures move through the lab, the number of operations going on simultaneously, and chain of custody.

In this Q & A, three experts from both academia and industry discuss the types of cell culture they perform in their labs, technologies they use, and the challenges they face.

As Canadian scholar Marshall McLuhan famously noted, “The medium is the message.” So it is with cell culture, where media and feed or supplementation strategies have been responsible for more improvement in cell productivity and performance than any other factors.

Isolating facility and layout from equipment during planning and maintenance of a cell culture lab
entails considerable risk, if for no other reason than the latter must fit into the former.