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LC biopharma environmental food and beverage compendium

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Thermo Fisher Scientific

Develop and establish workflows with more efficient use of resources

Overcome Your Lab&#39;s Resource Limitations

Whitepaper

Gene therapy concept. Medical technology. Medtech.

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Flexible and scalable nonviral delivery for cGMP cell therapy processing

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Figure 3. Gating strategy for the Attune NxT Flow Cytometer.

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Figure 5. Gene editing efficiency. The dark blue bar represents the percent of cells expressing the integrated CAR sequence (knock-in), light blue represents knockout, and grey represents the unedited or wild type population

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Figure 4. Cell viability 72 hours post-electroporation in the CTS Xenon system.

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Figure 6. Phenotypic data post-electroporation. The dark blue bar represents CD4? cells, the light blue bar represents CD8? cells, and the grey bar represents either double positive or double negative CD4/8 cells. Below the bar charts is phenotypic characterization before activation, electroporation, and culture

Figure 1. Autologous T cell therapy workflow.

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Figure 2. Experimental conditions and electroporation parameters.

Scale and Optimize Your Cell Therapy Processing

two scientists in a clinical lab using a cell therapy system

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Large-Scale Nonviral Delivery for Cell Therapy

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Graphical depiction of a user-generated electroporation pulse profile.

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The effects of voltage, pulse width, and pulse number on transfection efficiency and post-electroporation viability as determined by design of experiment (DOE).

Viability in CAR T cells. Cells were assessed for viability after 72 hours using Trypan Blue Solution. For the three donors, cell viability on the CTS Xenon system ranged from 64.6% to 83.6%.

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Allowable electroporation parameters based on power and temperature limits of the instrument and chamber.

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Knockout and knock-in performance in CAR T cells. Cells were characterized after 72 hours as untransfected (TCR??+, gray), knocked out but not knocked in (TCR??–CAR–, light blue), or as successfully knocked out and knocked in (TCR??–CAR+, dark blue). Across all donors, successful knock-in percentages on the CTS Xenon system ranged from 21.9% to 45.6%, exceeding the values on the Neon system.

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Products that meet your need at every step of the workflow, from research through commercial manufacturing

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Preservation of CD4 vs. CD8 T cell ratio. CD4 and CD8 T cells were identified by flow cytometry. Non-electroporated cells (condition 0) were gated on live, single, and untransfected (TCR??+ cells), while electroporated cells were gated on live, single, and knocked out (TCR??– cells). Proportions of CD4 (dark blue) and CD8 (light blue) T cells remained largely consistent between non-electroporated cells and cells electroporated with the Neon system (100 ?L) and with the CTS Xenon system (1 and 9 mL).

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Large-scale nonviral delivery for cell therapy

Flexible Solutions that Facilitate Scale-Up&nbsp;

Genetic engineering concept. Medical science. Scientific Laboratory.

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Automated PBMC isolation and T cell wash and concentration

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Figure 1. Simplified cell therapy workflow.

Figure 2. The CTS Rotea Counterflow Centrifugation System.

Figure 3. PBMC isolation using the CTS Rotea system. (A) Viability and recovery of PBMCs and (B) relative proportions of cell types present in an unprocessed Leukopak bag and after PBMC isolation.

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Figure 6. Average recovery and viability for two T cell wash and concentrate runs with an input of 5 x 10? cells.

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Table 3. T cell wash and concentrate protocol.

Figure 4. T cell expansion. (A) Expansion of T cells isolated using the CTS Rotea system and a density gradient medium. (B) Characterization of the expanded T cells isolated using the CTS Rotea system and a density gradient medium.

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Figure 7. T cell washing and concentration. (A) T cell recovery and viability averaged over ten T cell washing and concentration runs. (B) Flow cytometry after staining of CD4 and CD8, before and after the CTS Rotea system washing and concentration protocol. (C) CTS Rotea system operating software with a chamber of T cells in a fluidized bed.

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Figure 5. Recovery and viability for T cell washing and concentration with 5 mL, 10 mL, and 20 mL output volumes.

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Improve Your Process and Manufacturing in Cell and Gene Therapy

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A Closed Cell-Processing System for Cell Therapy manufacturing

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Processing times and instrument specifications chart

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What the CTS Rotea system does at each step

Cell separation, concentration, washing, and more

Are You Looking to Improve Your Closed Cell Processing?

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a photo of the cover of the vaccine development ebook

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Sartorius

Lab Manager

Streamlining Vaccine Research and Development

Resource Guide

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Lab technician placing sample inside of a bag.

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Lab technician loading samples into a centrifuge.

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A closeup of the top of a building on the Duke University School of Medicine–RTP Campus

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Managers and staff from Duke University&rsquo; Moody Lab learned many lessons from starting a COVID-19 testing program and relocating during the COVID-19 pandemic 

Research Lab Shifts Focus, Moves to New Lab Space During Pandemic

Labs Less Ordinary

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The projected global market for genomics from 2021 to 2026

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An infographic showing various next-generation sequencing choices and comparing their source, cost, and speed

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Genetic sequencing identifies overlooked approaches to therapy and treatment

Genomics Brings Precision to Medicine

Insights

A man riding a bike in a bike race for which he will need to take tramadol for his acute pain

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Fully automated dried spot analysis for the rapid quantitation of tramadol and its metabolites in various matrices

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Rapid quantitation of tramadol and its metabolites

Fully Automate Your Dry Spot Sampling and Analysis

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Call for Speakers for Lab Manager's virtual events and conferences

Lab Manager seeks expert speakers for upcoming virtual conferences

Call for Speakers - Lab Manager Digital Summits

Digital Event

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AI & Automation

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SciSure

Making Sense of Maximum Allowable Quantities

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Waters Corporation

Building and Sustaining Laboratory Compliance

Chemical Inventory Management Resource Guide Thumb

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Restoring Control Over Chemical Inventory

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Clinical

Overcome Your Lab's Resource Limitations