Protein Quantification
An acoustic ejection mass spectrometry system delivers significantly greater throughput and efficiency in screening and quantitation assays
As laboratories and facilities continue to elevate their technology and training, it is clear that qPCR will help push microbial diagnostics into new and fertile territory
Biochemical assays are dependent on the fidelity with which one can quantify the relative concentrations of proteins in solution.
Cell culture media fuel modern life sciences research. Whether the objective is cell-based assays, antibody production, or virus replication, the medium is a make-or-break component
Problem: Alterations in the proteome—including changes in protein expression, function, or localization—play a significant role in the development of cancer and other diseases.
Problem: Nearly 40 years after its introduction, western blotting continues to be a powerful method for protein quantitation. Arguably, finding the right antibody to detect the protein of interest is the technique’s most critical and challenging step.
Findings show that method can be a powerful complementary technique for assessing biosimilars
Stain-free gel chemistry uses a unique compound, which, when activated, reacts with tryptophan residues in the protein sample to emit a fluorescence signal. This allows the quick visualization of proteins without any staining steps. In addition, stain-free gel chemistry makes it possible to use total protein levels as a loading control rather than the housekeeping proteins used in traditional western blotting protocols. This negates the need to strip and reprobe blots and prevents any attendant errors that can be introduced at this step.
