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Choosing an in vitro model system that faithfully represents the natural physiology of the cell being studied is fundamental to understanding its in vivo function. Cultures of primary cell isolates retain their physiology and karyotype after isolation, but cultures may be difficult to prepare and are susceptible to contamination.

Basic research and clinical research labs have long relied on real-time PCR (qPCR) for its speed, sensitivity, specificity and ease-of-use. Common applications include gene expression analysis, mutation detection and identification of copy number variation to better understand inherited disorders, cancer and infectious disease.











