Dieter C. Gruenert, PhD, (bottom left) is a professor of otolaryngology—head and neck surgery at the University of California, San Francisco (UCSF) and pediatrics at the University of Vermont (UVM). He has a PhD in biophysics from the University of California at Berkeley and was a postdoctoral fellow in carcinogenesis at L’Institut Suisse de Recherche Expérimentale sur le Cancer in Lausanne. He was co- director of the Gene Therapy Core Center at UCSF, director of the Division of Human Molecular Genetics at  UVM, and head of the Stem Cell Research Program at California Pacific Medical Center. His research focuses on development of gene editing and cell-based therapies for inherited diseases and cancer. He has developed novel diagnostic and oligonucleotide-based therapeutic strategies to ameliorate disease pathology.

Dr. Donald Sakaguchi, professor of genetics, development and cell biology at Iowa State University, talks to contributing editor Dr. Tanuja Koppal about the time and cost savings generated in his lab due to a recent investment in a high-content screening (HCS) system. While his research group can now set up more experiments to look at different cell types and conditions, they also spend more time carefully designing the experiments and optimizing the conditions and later analyzing the vast amounts of data generated from each experiment.

Douglas Kiehl is a principal research scientist at Eli Lilly & Company, and is currently leader for the Spectroscopy & Raw Materials team. His group performs characterization and structural elucidation of impurities, related substances, and contaminants as well as testing and qualification of process raw materials through development and commercialization. Additionally, his team leads Lilly’s technical strategy for evaluating extractables and leachables associated with pharmaceutically relevant materials. 

Harm Moes is technical support engineer at SGS in the Netherlands. Mr. Moes has 13 years of experience in analytical instrumentation and held several technical positions with an analytical instrument supplier before joining SGS. At SGS, he provides technical support to the SGS oil, gas, and chemical labs in the Netherlands. That includes implementation and validation of new instrumentation, techniques, and methods, and instruction and training of laboratory personnel.

Dr. Nathaniel Hentz is assistant director of the analytical lab at the Golden LEAF Biomanufacturing Training and Education Center (BTEC), where he develops bioanalytical assays in support of the various biomanufacturing processes taught at BTEC. Since 2008, Dr. Hentz has been responsible for developing and teaching undergraduate and graduate courses, industry short courses, and government (FDA and BARDA) training courses, with a focus on assay development and validation, quality control, and liquid-handling performance.

Karyn M. Usher is an analytical chemist in the Department of Natural Sciences at Metropolitan State University in Saint Paul, MN. At Metro State, her research has focused on sample preparation for the determination of analytes in complex matrices by high performance liquid chromatography.

Dr. Guido Verbeck discusses recent developments in mass spectrometry with contributing writer Tanuja Koppal, PhD

Dr. Anne Carpenter leads the Imaging Platform at the Broad Institute of Harvard and MIT—a team of biologists and computer scientists who develop image analysis and data mining methods and software that are freely available to the public through the open-source CellProfiler project.

Dr. Arvind Rao has been an assistant professor in the Department of Bioinformatics and Computational Biology at the University of Texas MD Anderson Cancer Center since 2011.

Greg Martin is president of Complectors Consulting (www.complectors.com), based in Pottstown, PA, which provides consulting and training in the area of pharmaceutical analytical chemistry. Mr. Martin has over 25 years of experience in the pharmaceutical industry and was director of pharmaceutical analytical chemistry (R&D) for a major PhRMA company for a number of years. In addition, he has volunteered for the U.S. Pharmacopeia for over 10 years, and currently serves as vice chair of the General Chapters—Physical Analysis Expert Committee and also serves on expert panels on Validation and Verification; Weights and Balances’ Residual Solvents; and Use of Enzymes for Dissolution Testing of Gelatin Capsules.

Linda Wegley Kelly, PhD, a marine microbial ecologist in the Department of Biology at San Diego State University, talks to contributing editor Tanuja Koppal, PhD, about what has changed in the field since 2001, when she started working in the lab. While genomic and sequencing technologies have become easier and cheaper, the work on the bioinformatics side has now become more tedious in terms of the volume of data that needs to be analyzed. While systems for sample collection and storage have become convenient and customizable, the use of automation in microbiology remains fairly limited. Contamination still remains a cause for concern, and protocols have to be rigorously outlined and implemented.

Forest Rohwer, PhD, is a professor in the Department of Biology at San Diego State University. He is a fellow of the American Academy for Advancement of Science (AAAS), American Academy of Microbiology
(AAM), and Canadian Institute for Advanced Research (CIFAR). He led the development of viromics,” which involves isolating and sequencing the RNA/DNA from all the viruses in a sample. From this data, it is possible to determine what types of viruses are present and what functions they are encoding. Dr. Rohwer uses viromics to study ecosystems ranging from coral reefs to the human body and has shown that most genomic diversity on the planet is viral. Dr. Rohwer has published more than 150 peer-reviewed articles, was awarded the International Society of Microbial Ecology Young Investigators Award in 2008, and is listed as one of the World’s Most Influential Scientific Minds (Thomson Reuters 2014). 

Reginald Beer, PhD, medical diagnostics initiative leader at Lawrence Livermore National Laboratory, talks to contributing editor Tanuja Koppal, PhD, about the trends and innovations in digital PCR. While touting the advantages of digital PCR, he explains that not every lab needs to invest in this technology. Lab managers should look closely at their samples and assays to determine if digital PCR is needed for their application.