In order to maintain proper containment, NSF stipulates that recirculating and exhausted airfl ow volumes, and therefore velocities, must be maintained within a tolerance of +/- 5 feet per minute (FPM).
Goal:Optimize a UHPLC/MS method with respect to stationary phase, mobile phase, and detector settings to achieve picogram level quantitation of 14 drugs and metabolites employing a 12 minutes separation.