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Large-Scale Nonviral Delivery for Cell Therapy

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Graphical depiction of a user-generated electroporation pulse profile.

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The effects of voltage, pulse width, and pulse number on transfection efficiency and post-electroporation viability as determined by design of experiment (DOE).

Viability in CAR T cells. Cells were assessed for viability after 72 hours using Trypan Blue Solution. For the three donors, cell viability on the CTS Xenon system ranged from 64.6% to 83.6%.

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Allowable electroporation parameters based on power and temperature limits of the instrument and chamber.

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Knockout and knock-in performance in CAR T cells. Cells were characterized after 72 hours as untransfected (TCR??+, gray), knocked out but not knocked in (TCR??–CAR–, light blue), or as successfully knocked out and knocked in (TCR??–CAR+, dark blue). Across all donors, successful knock-in percentages on the CTS Xenon system ranged from 21.9% to 45.6%, exceeding the values on the Neon system.

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Products that meet your need at every step of the workflow, from research through commercial manufacturing

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Preservation of CD4 vs. CD8 T cell ratio. CD4 and CD8 T cells were identified by flow cytometry. Non-electroporated cells (condition 0) were gated on live, single, and untransfected (TCR??+ cells), while electroporated cells were gated on live, single, and knocked out (TCR??– cells). Proportions of CD4 (dark blue) and CD8 (light blue) T cells remained largely consistent between non-electroporated cells and cells electroporated with the Neon system (100 ?L) and with the CTS Xenon system (1 and 9 mL).

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Thermo Fisher Scientific

Large-scale nonviral delivery for cell therapy

Flexible Solutions that Facilitate Scale-Up&nbsp;

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XiltriX

The ultimate guide to always ensuring a controlled lab environment

Laboratory Environmental Monitoring

Resource Guide

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A turn-of-the-century chocolate factory finds new life as a lab

Project Profile: Sema4 Clinical Laboratory

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COVID-19 Resource Guide

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Expert advice on how to manage your lab during a pandemic

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Move Beyond Policies to Build a Lab Culture Where Safety is Second Nature

Turning Safety Principles Into Daily Practice

End-to-end workflow from liquid biopsy sample to data analysis

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The Agilent Avida Duo workflow enables the detection of DNA variants and methylation markers from the same sample input, with a fast turnaround time. 

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Initiate your analysis from FASTQ files from LBx or methylation samples

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End-to-end workflow from liquid biopsy sample to data analysis. 

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Agilent Technologies

Multiomic Insights from Liquid Biopsy with Agilent Avida and SeqOne Platform

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PerkinElmer

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SCIEX

Maximizing the Performance and Lifespan of Analytical Equipment

Rusty Bishop, PhD, chief commercial officer at TubeWriter.

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Flexible Solutions that Facilitate Scale-Up

Laboratory Environmental Monitoring

Project Profile: Sema4 Clinical Laboratory

COVID-19 Resource Guide

Turning Safety Principles Into Daily Practice

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Multiomic Insights from Liquid Biopsy with Agilent Avida and SeqOne Platform

Maximizing the Performance and Lifespan of Analytical Equipment

Replacing Stickers with Laboratory-Grade Labeling

Accurate and Reliable Elemental Speciation with PerkinElmer