High-performance liquid chromatography (HPLC) columns are considered the “heart” of the instrument because that is where the separations occur. Columns consist of stainless steel tubes with inlet and outlet openings. Plastic or glass may
Chromatographers who learned their craft twenty years ago may not have been familiar with autosamplers then, but today nearly every high performance liquid chromatography(HPLC) instrument includes an autosampler as standard equipment.
High performance liquid chromatography has been one of the defining separation techniques of the last 40 years, and its importance and range of uses will likely increase in the coming years.
Chromatographic separation methods can be developed on any scale. To minimize consumption of sample and solvents there is a benefit in developing separation methods on a small scale and transferring them to a larger scale.
The origins of HPLC date back to the invention of chromatography in the early 20th century, through the introduction of partition and paper chromatography in the 1940s, to the introduction of liquid chromatography in the early 1960s.
David Ji, laboratory director at Analytical Laboratories in Anaheim, Inc., talks about the different ways in which his service laboratory uses chromatography techniques to analyze complex sample mixtures for various clients.
Among the hyphenated mass spectrometry (MS) techniques, gas chromatography-MS (GC-MS) and high-performance liquid chromatography- MS (LC-MS) are the most notable. These methods combine the resolving power of high-resolution chromatography with the specificity and sensitivity of MS.
Dolomite, in collaboration with the UKs National Centre for Atmospheric Science, has successfully tested the miniaturization of gas chromatography equipment for environmental testing.