The origins of high-performance liquid chromatography (HPLC) date back to the invention of chromatography in the early 20th century, then to partition and paper chromatography in the 1940s, and finally to the introduction of liquid chromatography in the early 1960s.

High-performance liquid chromatography (HPLC) columns are considered the “heart” of the instrument used to transport the analyte and the mobile phase and provide the environment in which separation is achieved. 

UHPLC – ultra high performance liquid chromatography – systems have been around since 2003 and continue to grow. These systems, while having better performance than traditional HPLC, have more limited surface chemistries than HPLC.

Our six experts from both industry and acadmia provide their thoughts on HPLC systems in this Q&A, commenting on which systems work best for them, and more.

Detection of carbendazim residue at concentrations below 10 PPB is possible without sample preparation or pre-concentration

hermo Fisher Scientific is pleased to announce high performance liquid chromatography (HPLC) and ultra HPLC (UHPLC) methods that can be used to assay tetracycline (TC) and 4 epianhydrotetracycline (EATC) in a TC hydrochloride (HCl) capsule.

Thermo Fisher Scientific is pleased to announce an on-line solidphase extraction (SPE) high-performance liquid chromatography(HPLC) system that speeds and accelerates the determination of aniline and nitroanilines in tap and pond water.

Nowhere is the controversy over instrument upgrades more animated than in HPLC, or more correctly, in the debate over switching from HPLC to UHPLC.