Chromatographers who learned their craft twenty years ago may not have been familiar with autosamplers then, but today nearly every high performance liquid chromatography(HPLC) instrument includes an autosampler as standard equipment.
Chromatographers who learned their craft twenty years ago may not have been familiar with autosamplers then, but today nearly every high performance liquid chromatography (HPLC) instrument includes an autosampler as standard equipment.
Chromatographic separation methods can be developed on any scale. To minimize consumption of sample and solvents there is a benefit in developing separation methods on a small scale and transferring them to a larger scale.
The origins of HPLC date back to the invention of chromatography in the early 20th century, through the introduction of partition and paper chromatography in the 1940s, to the introduction of liquid chromatography in the early 1960s.
Among the hyphenated mass spectrometry (MS) techniques, gas chromatography-MS (GC-MS) and high-performance liquid chromatography- MS (LC-MS) are the most notable. These methods combine the resolving power of high-resolution chromatography with the specificity and sensitivity of MS.