Sample Preparation

Mechanical sample lysis is rapidly becoming the preferred sample preparation method in life science labs for the isolation of DNA, RNA, proteins, metabolites and other small molecules because the elimination of chemicals, enzymes, and detergents minimizes the introduction of potential inhibitors to downstream processes. The FastPrep-24 5G™ (Fig. 1) is the newest innovation in beat-beaters and produces the fastest lysis of even the most difficult samples.

Thermo Fisher Scientific has developed a rapid and reliable sample preparation procedure and ion chromatography-based method to determine the phytate hydrolysis products in dried distillers grains with
solubles (DDGS).

Problem: With unrelenting need for accurate sample analysis at lower and lower detection limits, there is pressure on modern laboratories for sample prep instruments that can provide automated, accurate reagent additions to previously prepared liquid samples or for preparing several aliquots of these samples with multiple dilution factors. The catch-all phrase that identifies these devices is “liquid handling systems” and they perform absolutely essential tasks that have a direct and large effect on the ultimate measurement accuracy of both inorganic and organic sample analysis.

Problem: Bringing samples into a mineralized state is usually the first step for performing accurate inorganic analysis, aka metals trace analysis. This conversion of a usually non-homogenous insoluble mixture into a representative, homogenous aqueous solution using heat and acids is usually a prerequisite for further analysis work with spectroscopic or chromatographic techniques.










