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With the right filter in dissolution testing, you can cut instrument maintenance costs, reduce time-consuming retests, and gain confidence in your results.

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Cytiva UK Limited

Cytiva

&nbsp;Why careful membrane and media selection is a crucial step in the dissolution testing workflow

How The Right Filter Can Save Your Lab Time and Money

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Gene therapy concept. Medical technology. Medtech.

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Flexible and scalable nonviral delivery for cGMP cell therapy processing

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Figure 3. Gating strategy for the Attune NxT Flow Cytometer.

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Figure 5. Gene editing efficiency. The dark blue bar represents the percent of cells expressing the integrated CAR sequence (knock-in), light blue represents knockout, and grey represents the unedited or wild type population

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Figure 4. Cell viability 72 hours post-electroporation in the CTS Xenon system.

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Figure 6. Phenotypic data post-electroporation. The dark blue bar represents CD4? cells, the light blue bar represents CD8? cells, and the grey bar represents either double positive or double negative CD4/8 cells. Below the bar charts is phenotypic characterization before activation, electroporation, and culture

Figure 1. Autologous T cell therapy workflow.

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Figure 2. Experimental conditions and electroporation parameters.

Thermo Fisher Scientific

Scale and Optimize Your Cell Therapy Processing

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Large-Scale Nonviral Delivery for Cell Therapy

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Graphical depiction of a user-generated electroporation pulse profile.

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The effects of voltage, pulse width, and pulse number on transfection efficiency and post-electroporation viability as determined by design of experiment (DOE).

Viability in CAR T cells. Cells were assessed for viability after 72 hours using Trypan Blue Solution. For the three donors, cell viability on the CTS Xenon system ranged from 64.6% to 83.6%.

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Allowable electroporation parameters based on power and temperature limits of the instrument and chamber.

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Knockout and knock-in performance in CAR T cells. Cells were characterized after 72 hours as untransfected (TCR??+, gray), knocked out but not knocked in (TCR??–CAR–, light blue), or as successfully knocked out and knocked in (TCR??–CAR+, dark blue). Across all donors, successful knock-in percentages on the CTS Xenon system ranged from 21.9% to 45.6%, exceeding the values on the Neon system.

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Products that meet your need at every step of the workflow, from research through commercial manufacturing

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Preservation of CD4 vs. CD8 T cell ratio. CD4 and CD8 T cells were identified by flow cytometry. Non-electroporated cells (condition 0) were gated on live, single, and untransfected (TCR??+ cells), while electroporated cells were gated on live, single, and knocked out (TCR??– cells). Proportions of CD4 (dark blue) and CD8 (light blue) T cells remained largely consistent between non-electroporated cells and cells electroporated with the Neon system (100 ?L) and with the CTS Xenon system (1 and 9 mL).

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Large-scale nonviral delivery for cell therapy

Flexible Solutions that Facilitate Scale-Up&nbsp;

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Closed, Automated Wash and Concentration of Expanded Human Natural Killer (NK) Cells

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Figure 4. NK cell washing and concentration.

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Figure 6. Maintenance of NK cell cytotoxicity after washing and concentration.

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Figure 3. Fold expansion of NK cells cultured in CTS NK-Xpander Medium for 17 days.

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Figure 3. Fold expansion of NK cells cultured in CTS NK-Xpander Medium for 17 days

Figure 1. CTS Rotea Counterflow Centrifugation System and CTS NK-Xpander Medium.

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Figure 5. Maintenance of NK cell degranulation capability after washing and concentration.

Closed, automated washing and concentration of expanded human natural killer cells

Increase Efficiency in Your Cell Processing&nbsp;

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Automated PBMC isolation and T cell wash and concentration

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Figure 1. Simplified cell therapy workflow.

Figure 2. The CTS Rotea Counterflow Centrifugation System.

Figure 3. PBMC isolation using the CTS Rotea system. (A) Viability and recovery of PBMCs and (B) relative proportions of cell types present in an unprocessed Leukopak bag and after PBMC isolation.

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Figure 6. Average recovery and viability for two T cell wash and concentrate runs with an input of 5 x 10? cells.

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Table 3. T cell wash and concentrate protocol.

Figure 4. T cell expansion. (A) Expansion of T cells isolated using the CTS Rotea system and a density gradient medium. (B) Characterization of the expanded T cells isolated using the CTS Rotea system and a density gradient medium.

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Figure 7. T cell washing and concentration. (A) T cell recovery and viability averaged over ten T cell washing and concentration runs. (B) Flow cytometry after staining of CD4 and CD8, before and after the CTS Rotea system washing and concentration protocol. (C) CTS Rotea system operating software with a chamber of T cells in a fluidized bed.

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Figure 5. Recovery and viability for T cell washing and concentration with 5 mL, 10 mL, and 20 mL output volumes.

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Improve Your Process and Manufacturing in Cell and Gene Therapy

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A Closed Cell-Processing System for Cell Therapy manufacturing

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Processing times and instrument specifications chart

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What the CTS Rotea system does at each step

Cell separation, concentration, washing, and more

Are You Looking to Improve Your Closed Cell Processing?

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Porvair Sciences

Novel hybrid technology improves phospholipid removal from plasma/sera

Obtain More Reproducible Data

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BioChromato

Research, development, and quality control

Detecting Early-Stage Degradation in Engineering Polymers

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Lab Companion SC3 Fire Safety Storage Cabinet

Fire Safety Cabinet

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Jeio Tech

Lab Manager

A manufacturer&rsquo;s integrated capacity is critical to ensure reliability

Building a Lab with High-Quality Equipment

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Agilent Technologies

Advances in Food Testing &amp; Environmental Analysis

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Food Testing &amp; Environmental Analysis

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Slingshot Biosciences

Replacing biologically-derived flow controls with synthetic cells to improve traceability and data precision

Synthetic Cells: Changing the Face of Flow Cytometry

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How Lab Leaders Can Prepare for Safety Crises That Don’t Follow the Script

When the Unexpected Hits

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Lab Manager Academy | Essential training that every lab manager needs to succeed.

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Lab Manager Academy

The Power of Persuasion

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PolyScience

Verify Circulator Performance without Waiting for Service

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Ansell

Kimtech&trade; Polaris&trade; Nitrile Exam Gloves. Built For Science. Made For You.

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Whitepaper

How The Right Filter Can Save Your Lab Time and Money

Scale and Optimize Your Cell Therapy Processing

Flexible Solutions that Facilitate Scale-Up

Increase Efficiency in Your Cell Processing

Improve Your Process and Manufacturing in Cell and Gene Therapy

Are You Looking to Improve Your Closed Cell Processing?

Obtain More Reproducible Data

Detecting Early-Stage Degradation in Engineering Polymers

Building a Lab with High-Quality Equipment

Advances in Food Testing & Environmental Analysis

Food Testing & Environmental Analysis

Synthetic Cells: Changing the Face of Flow Cytometry

When the Unexpected Hits

Sponsored

The Power of Persuasion

Verify Circulator Performance without Waiting for Service

Kimtech™ Polaris™ Nitrile Exam Gloves. Built For Science. Made For You.

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